Detailed information of TA system TA217657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 647137..647936
• Replicon: chromosome
• Accession: NZ_CP083877
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: LCE22_RS03155
• Protein ID: WP_000347272.1
• Coordinates: 647137..647601 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: LCE22_RS03160
• Protein ID: WP_001307405.1
• Coordinates: 647601..647936 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE22_RS03125 (642138)	642138..642572	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LCE22_RS03130 (642590)	642590..643468	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
LCE22_RS03135 (643458)	643458..644237	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LCE22_RS03140 (644248)	644248..644721	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LCE22_RS03145 (644744)	644744..646024	-	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LCE22_RS03150 (646273)	646273..647082	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
LCE22_RS03155 (647137)	647137..647601	-	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LCE22_RS03160 (647601)	647601..647936	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LCE22_RS03165 (648085)	648085..649656	-	1572	WP_001273738.1	galactarate dehydratase	-
LCE22_RS03170 (650031)	650031..651365	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
LCE22_RS03175 (651381)	651381..652151	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	647137..658589	11452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T217657 WP_000347272.1 NZ_CP083877:c647601-647137 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T217657 NZ_CP114551:c1015901-1015605 [Xylella fastidiosa subsp. pauca]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCACGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCAGCGTGGCGACACGATCTACTTGCTACTTTGCGGCGGTGACAAAGGA
TTACAAGCGCGTGACATCAAGACTGCGCTGCACCTCTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT217657 WP_001307405.1 NZ_CP083877:c647936-647601 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT217657 NZ_CP114551:c1015602-1015309 [Xylella fastidiosa subsp. pauca]
ATGACCGAGAAACTGACGAGCTACGATCCGGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCCGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTAGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTAGCC
GTGATGAAGGCGCTAGGGATCGAGCTCTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7