Detailed information of TA system TA217651

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 114607..115028
• Replicon: plasmid unnamed3
• Accession: NZ_CP083875
• Organism: Escherichia coli strain NDM5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE27_RS23885
• Protein ID: WP_096937776.1
• Coordinates: 114607..114732 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	114830..115028 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE27_RS23845 (109671)	109671..109886	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
LCE27_RS23850 (110022)	110022..110669	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
LCE27_RS23855 (110860)	110860..111243	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE27_RS23860 (111613)	111613..112185	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
LCE27_RS23865 (112481)	112481..113302	-	822	WP_001234465.1	DUF932 domain-containing protein	-
LCE27_RS23870 (113421)	113421..113708	-	288	WP_000107242.1	hypothetical protein	-
LCE27_RS23875 (113733)	113733..113939	-	207	WP_000275854.1	hypothetical protein	-
LCE27_RS23880 (114009)	114009..114306	+	298	Protein_135	hypothetical protein	-
LCE27_RS23885 (114607)	114607..114732	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE27_RS23890 (114674)	114674..114823	-	150	Protein_137	DUF5431 family protein	-
- (114830)	114830..115028	-	199	NuclAT_0	-	Antitoxin
- (114830)	114830..115028	-	199	NuclAT_0	-	Antitoxin
- (114830)	114830..115028	-	199	NuclAT_0	-	Antitoxin
- (114830)	114830..115028	-	199	NuclAT_0	-	Antitoxin
LCE27_RS23895 (114997)	114997..115102	-	106	Protein_138	plasmid SOS inhibition protein A	-
LCE27_RS23900 (115257)	115257..115574	+	318	Protein_139	IS110 family transposase	-
LCE27_RS23905 (115863)	115863..116114	-	252	Protein_140	iron-containing alcohol dehydrogenase	-
LCE27_RS23910 (116199)	116199..116711	-	513	WP_223350513.1	potassium channel family protein	-
LCE27_RS23915 (116838)	116838..117531	-	694	Protein_142	IS1 family transposase	-
LCE27_RS23920 (117588)	117588..117692	+	105	Protein_143	thiosulfate reductase cytochrome B subunit	-
LCE27_RS23925 (117938)	117938..119332	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / dfrA12 / aadA2 / qacE / sul1	-	1..170625	170625
	-	flank	IS/Tn	-	-	116838..117215	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T217651 WP_096937776.1 NZ_CP083875:c114732-114607 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T217651 NZ_CP114550:2352321-2352623 [Xylella fastidiosa subsp. pauca]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTAAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT217651 NZ_CP083875:c115028-114830 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin