Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2178022..2178524 Replicon chromosome
Accession NC_008563
Organism Escherichia coli APEC O1

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag APECO1_RS11220 Protein ID WP_000767826.1
Coordinates 2178022..2178276 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag APECO1_RS11225 Protein ID WP_001259255.1
Coordinates 2178273..2178524 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO1_RS11195 2173037..2173264 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
APECO1_RS11200 2173278..2174336 - 1059 WP_000492334.1 transport protein YeeE -
APECO1_RS11205 2174515..2175873 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
APECO1_RS31125 2175863..2175925 - 63 WP_010723108.1 membrane protein YoeI -
APECO1_RS11210 2176140..2177069 - 930 WP_000803363.1 LysR family transcriptional regulator -
APECO1_RS11215 2177115..2177939 - 825 WP_000754737.1 SDR family oxidoreductase -
APECO1_RS11220 2178022..2178276 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
APECO1_RS11225 2178273..2178524 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
APECO1_RS31130 2178807..2178848 + 42 WP_010989201.1 his operon leader peptide -
APECO1_RS11230 2178994..2179893 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
APECO1_RS11235 2179899..2181203 + 1305 WP_001350692.1 histidinol dehydrogenase -
APECO1_RS11240 2181200..2182270 + 1071 WP_000108997.1 histidinol-phosphate transaminase -
APECO1_RS11245 2182270..2183337 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T21765 WP_000767826.1 NC_008563:c2178276-2178022 [Escherichia coli APEC O1]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T21765 NC_008563:c2178276-2178022 [Escherichia coli APEC O1]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT21765 WP_001259255.1 NC_008563:c2178524-2178273 [Escherichia coli APEC O1]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT21765 NC_008563:c2178524-2178273 [Escherichia coli APEC O1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References