Detailed information of TA system TA217649

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74602..74856
• Replicon: plasmid unnamed3
• Accession: NZ_CP083875
• Organism: Escherichia coli strain NDM5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LCE27_RS23645
• Protein ID: WP_001312851.1
• Coordinates: 74602..74751 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	74795..74856 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE27_RS23615 (69849)	69849..70760	-	912	WP_000440183.1	carbamate kinase	-
LCE27_RS23620 (70771)	70771..71991	-	1221	WP_000410951.1	arginine deiminase	-
LCE27_RS23625 (72698)	72698..73312	+	615	Protein_84	VENN motif pre-toxin domain-containing protein	-
LCE27_RS23630 (73312)	73312..73758	-	447	Protein_85	plasmid replication initiator RepA	-
LCE27_RS23635 (73751)	73751..73825	-	75	WP_032336874.1	RepA leader peptide Tap	-
LCE27_RS23640 (74061)	74061..74318	-	258	WP_000083833.1	replication regulatory protein RepA	-
LCE27_RS23645 (74602)	74602..74751	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (74795)	74795..74856	+	62	NuclAT_1	-	Antitoxin
- (74795)	74795..74856	+	62	NuclAT_1	-	Antitoxin
- (74795)	74795..74856	+	62	NuclAT_1	-	Antitoxin
- (74795)	74795..74856	+	62	NuclAT_1	-	Antitoxin
LCE27_RS23650 (74995)	74995..75177	-	183	WP_000968309.1	hypothetical protein	-
LCE27_RS23655 (75278)	75278..75894	+	617	Protein_90	IS1-like element IS1A family transposase	-
LCE27_RS23660 (75932)	75932..77503	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
LCE27_RS23665 (77523)	77523..77870	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
LCE27_RS23670 (77870)	77870..78547	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
LCE27_RS23675 (78602)	78602..78691	+	90	Protein_94	IS1 family transposase	-
LCE27_RS23680 (78992)	78992..79204	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / dfrA12 / aadA2 / qacE / sul1	-	1..170625	170625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T217649 WP_001312851.1 NZ_CP083875:c74751-74602 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T217649 NZ_CP114550:c1698999-1698718 [Xylella fastidiosa subsp. pauca]
ATGCGGAGGATTAGCCAGACTGGCCAGTTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTCATACACGTCGTGCATGCCTTAGCCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTGACTG
GCAGCTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCAGACGACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT217649 NZ_CP083875:74795-74856 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin