Detailed information of TA system TA217644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3670938..3671632
• Replicon: chromosome
• Accession: NZ_CP083872
• Organism: Escherichia coli strain NDM5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LCE27_RS17815
• Protein ID: WP_001263489.1
• Coordinates: 3670938..3671336 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LCE27_RS17820
• Protein ID: WP_000554758.1
• Coordinates: 3671339..3671632 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3666526)	3666526..3666606	-	81	NuclAT_15	-	-
- (3666526)	3666526..3666606	-	81	NuclAT_15	-	-
- (3666526)	3666526..3666606	-	81	NuclAT_15	-	-
- (3666526)	3666526..3666606	-	81	NuclAT_15	-	-
LCE27_RS17790 (3667202)	3667202..3667660	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LCE27_RS17795 (3667921)	3667921..3669378	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LCE27_RS17800 (3669435)	3669435..3669956	-	522	Protein_3478	peptide chain release factor H	-
LCE27_RS17805 (3669952)	3669952..3670158	-	207	Protein_3479	RtcB family protein	-
LCE27_RS17810 (3670476)	3670476..3670928	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LCE27_RS17815 (3670938)	3670938..3671336	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LCE27_RS17820 (3671339)	3671339..3671632	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LCE27_RS17825 (3671684)	3671684..3672739	-	1056	WP_001226164.1	DNA polymerase IV	-
LCE27_RS17830 (3672810)	3672810..3673595	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LCE27_RS17835 (3673567)	3673567..3675279	+	1713	Protein_3485	flagellar biosynthesis protein FlhA	-
LCE27_RS17840 (3675503)	3675503..3676000	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3602736..3676802	74066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T217644 WP_001263489.1 NZ_CP083872:c3671336-3670938 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217644 NZ_CP114550:c1217623-1217348 [Xylella fastidiosa subsp. pauca]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTGGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGACGAGAATGCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT217644 WP_000554758.1 NZ_CP083872:c3671632-3671339 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217644 NZ_CP114550:c1217834-1217607 [Xylella fastidiosa subsp. pauca]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTATCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3