Detailed information of TA system TA217632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1782529..1783031
• Replicon: chromosome
• Accession: NZ_CP083872
• Organism: Escherichia coli strain NDM5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LCE27_RS08510
• Protein ID: WP_000767832.1
• Coordinates: 1782777..1783031 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LCE27_RS08505
• Protein ID: WP_001259255.1
• Coordinates: 1782529..1782780 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE27_RS08480 (1777707)	1777707..1778774	-	1068	WP_000080064.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LCE27_RS08485 (1778774)	1778774..1779844	-	1071	WP_094336856.1	histidinol-phosphate transaminase	-
LCE27_RS08490 (1779841)	1779841..1781145	-	1305	WP_024198577.1	histidinol dehydrogenase	-
LCE27_RS08495 (1781151)	1781151..1782050	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LCE27_RS08500 (1782196)	1782196..1782246	-	51	WP_001364200.1	his operon leader peptide	-
LCE27_RS08505 (1782529)	1782529..1782780	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LCE27_RS08510 (1782777)	1782777..1783031	+	255	WP_000767832.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LCE27_RS08515 (1783114)	1783114..1783938	+	825	WP_000754737.1	SDR family oxidoreductase	-
LCE27_RS08520 (1783984)	1783984..1784913	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
LCE27_RS08525 (1785127)	1785127..1785189	+	63	WP_097433929.1	membrane protein YoeI	-
LCE27_RS08530 (1785179)	1785179..1786537	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10232.66 Da
• Isoelectric Point: 8.0280

>T217632 WP_000767832.1 NZ_CP083872:1782777-1783031 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHYLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T217632 NZ_CP114549:c1226289-1225678 [Xylella fastidiosa subsp. pauca]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGCCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCAATTTTTGTGTGGCCTTGATGCTGAGGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACAGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGCGGATTGGC
TAGTATCATCCGCGATGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT217632 WP_001259255.1 NZ_CP083872:1782529-1782780 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT217632 NZ_CP114549:c1226474-1226286 [Xylella fastidiosa subsp. pauca]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0