Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 619691..620490 Replicon chromosome
Accession NZ_CP083872
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag LCE27_RS03030 Protein ID WP_000347272.1
Coordinates 619691..620155 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCE27_RS03035 Protein ID WP_001307405.1
Coordinates 620155..620490 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS03000 (614692) 614692..615126 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCE27_RS03005 (615144) 615144..616022 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
LCE27_RS03010 (616012) 616012..616791 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCE27_RS03015 (616802) 616802..617275 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCE27_RS03020 (617298) 617298..618578 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCE27_RS03025 (618827) 618827..619636 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
LCE27_RS03030 (619691) 619691..620155 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCE27_RS03035 (620155) 620155..620490 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCE27_RS03040 (620639) 620639..622210 - 1572 WP_001273738.1 galactarate dehydratase -
LCE27_RS03045 (622585) 622585..623919 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LCE27_RS03050 (623935) 623935..624705 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 619691..631143 11452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T217628 WP_000347272.1 NZ_CP083872:c620155-619691 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T217628 NZ_CP114549:1196821-1197123 [Xylella fastidiosa subsp. pauca]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTGCTTTGCGGTGGCGATAAATCG
TCACAAATGCGAGATATCGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATGAGCCATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT217628 WP_001307405.1 NZ_CP083872:c620490-620155 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT217628 NZ_CP114549:1197120-1197425 [Xylella fastidiosa subsp. pauca]
ATGAATAATGAGACGTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTAGCTAAGGCGCTTGGTTTACGGATGTCATTCACTCCTTCCTCTATGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References