Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4253174..4253683 Replicon chromosome
Accession NZ_CP083869
Organism Escherichia coli strain NDM6

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag LCE24_RS22000 Protein ID WP_089514152.1
Coordinates 4253174..4253359 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LCE24_RS22005 Protein ID WP_000398619.1
Coordinates 4253387..4253683 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE24_RS21990 (4249858) 4249858..4251315 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LCE24_RS21995 (4251552) 4251552..4253069 + 1518 WP_001295074.1 lysine--tRNA ligase -
LCE24_RS22000 (4253174) 4253174..4253359 - 186 WP_089514152.1 type V toxin-antitoxin system toxin GhoT Toxin
LCE24_RS22005 (4253387) 4253387..4253683 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCE24_RS22010 (4253909) 4253909..4254181 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LCE24_RS22015 (4254193) 4254193..4254423 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LCE24_RS22020 (4254604) 4254604..4256234 + 1631 Protein_4035 sensor histidine kinase -
LCE24_RS22025 (4256231) 4256231..4256950 + 720 WP_224452708.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4244896..4253683 8787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-54)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6988.54 Da        Isoelectric Point: 10.2380

>T217626 WP_089514152.1 NZ_CP083869:c4253359-4253174 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFLSFRSCS

Download         Length: 186 bp

>T217626 NZ_CP114549:994830-995111 [Xylella fastidiosa subsp. pauca]
GTGCGGGAAATTAAACAACTCGGGCAGTTCAAACGTGACTTGAAGCGCGAAGCCAAGGGGCAGCATCGCGCCACGCTGGA
AGACGACCTGCTGCCCGTGATCGATGCCCTTGCTAAAGACATGCCCCTTGAGCCTCGGCACAGAGATCACGCACTGCTTG
GCAACTGGAAAGACCTTCGTGATTGTCACATCAAGCTCGACCTAGTCCTGATCTACACGCGGGTGGATAGCAAAACGCTG
ACGCTCGTCCGTCTCGGCTCCCATGCGGAACTCGGGCTGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT217626 WP_000398619.1 NZ_CP083869:c4253683-4253387 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217626 NZ_CP114549:994583-994843 [Xylella fastidiosa subsp. pauca]
ATGGCTGCGAATCAGCTTGTGCAAGCGCGCATCGATGGTGCGATCAAGGCAGAGGCAACCGTGGTACTGGCGGCGATGGG
GTTGACCATTTCCGATGCGGTTCGGCTGTTGCTCACGAAGGTGGCTCAGGACAAGGCGTTGCCTTTTGAGCCGCTAATCC
CTAATGCCACCACCATCAAGGCCATGCGGGAGGCTCGTGCAGGTAAGGGCGAGACCGTCACGCTGGGTGAACTTCGAGCG
ACTATCCGTGCGGGAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References