Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3703866..3704407 Replicon chromosome
Accession NZ_CP083869
Organism Escherichia coli strain NDM6

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LCE24_RS19325 Protein ID WP_000615983.1
Coordinates 3704129..3704407 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LCE24_RS19320 Protein ID WP_000729703.1
Coordinates 3703866..3704126 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE24_RS19300 (3699541) 3699541..3700326 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LCE24_RS19305 (3700298) 3700298..3702010 + 1713 Protein_3509 flagellar biosynthesis protein FlhA -
LCE24_RS19310 (3702234) 3702234..3702731 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LCE24_RS19315 (3702907) 3702907..3703656 - 750 WP_000056849.1 C40 family peptidase -
LCE24_RS19320 (3703866) 3703866..3704126 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LCE24_RS19325 (3704129) 3704129..3704407 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LCE24_RS19330 (3704563) 3704563..3705303 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LCE24_RS19335 (3705274) 3705274..3706041 - 768 WP_000333380.1 class II glutamine amidotransferase -
LCE24_RS19340 (3706247) 3706247..3706825 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T217623 WP_000615983.1 NZ_CP083869:3704129-3704407 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217623 NZ_CP114542:c2966575-2966210 [Brevundimonas subvibrioides]
ATGGTGAAGGCGCTGTTCGACACCAATATCCTGATCGATCATCTGAACGCCGCGCCGGAGGCCGATGCCGAGATCGATCG
GTACGATGACCGCGCGATCAGCATCATTACCTGGATCGAGGTCATGGCGGGTGCCGATGCCGAGCTGGCCGACCCAACCC
GGACCTATCTCGATGGATATTCGATCGTTGCTCTTGATGACCCGATCGCGGAGCGCGCGGCGTCGATACGCCAGTCTCGC
CGCATGAAGCTGCCTGACGCCATCATCTGGGCGACCGCACAGACGACGGGTCGTCTGCTGGTCACCCGCAACACCAAGGA
TTTTCCGGCTGACGATCCGGGGGTCCGGGCACCCTATGTTCTCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT217623 WP_000729703.1 NZ_CP083869:3703866-3704126 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217623 NZ_CP114542:c2966784-2966569 [Brevundimonas subvibrioides]
ATGCGTATCCTGGTGGATGCCAATGAGGAGCAGGTCGCTGCGCTGGACGCCCTGGCCAAACGCGAGAAGCGCCCCCGCGC
GGCCGTGATCCGCGCCGCGATCGACGATTACGTCGCGCGCAACAAGCGTCAGGCGGTCAAGGACGGTTTCGGTCTTTGGG
GCCAGGGCGGCGAAGACGGGCTTGCTTATCAGGAACGACTGCGTAGCGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References