Detailed information of TA system TA217622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3697669..3698363
• Replicon: chromosome
• Accession: NZ_CP083869
• Organism: Escherichia coli strain NDM6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LCE24_RS19285
• Protein ID: WP_001263489.1
• Coordinates: 3697669..3698067 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LCE24_RS19290
• Protein ID: WP_000554758.1
• Coordinates: 3698070..3698363 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3693257)	3693257..3693337	-	81	NuclAT_13	-	-
- (3693257)	3693257..3693337	-	81	NuclAT_13	-	-
- (3693257)	3693257..3693337	-	81	NuclAT_13	-	-
- (3693257)	3693257..3693337	-	81	NuclAT_13	-	-
LCE24_RS19260 (3693933)	3693933..3694391	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LCE24_RS19265 (3694652)	3694652..3696109	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LCE24_RS19270 (3696166)	3696166..3696687	-	522	Protein_3502	peptide chain release factor H	-
LCE24_RS19275 (3696683)	3696683..3696889	-	207	Protein_3503	RtcB family protein	-
LCE24_RS19280 (3697207)	3697207..3697659	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LCE24_RS19285 (3697669)	3697669..3698067	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LCE24_RS19290 (3698070)	3698070..3698363	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LCE24_RS19295 (3698415)	3698415..3699470	-	1056	WP_001226164.1	DNA polymerase IV	-
LCE24_RS19300 (3699541)	3699541..3700326	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LCE24_RS19305 (3700298)	3700298..3702010	+	1713	Protein_3509	flagellar biosynthesis protein FlhA	-
LCE24_RS19310 (3702234)	3702234..3702731	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T217622 WP_001263489.1 NZ_CP083869:c3698067-3697669 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217622 NZ_CP114542:c2881253-2880927 [Brevundimonas subvibrioides]
ATGTCGCCCATAGACGACAGTTCTGGCTTCGAGCTCGAGTTCTCGGAGACGTTCGATGCATGGCTTCAAGGGCTCCGTGA
TCACCGCGCTGCCGCGCGTATCACGGTACGGCTTGAAAGACTTGAGCGTGGTCATTGGGGCGACGCCAGGTCTGTCGGCG
AGGGCGTAACGGAGCTGCGTATCGATTATGGCCCCGGCTACCGGCTCTACGCCGCGCGGAGGGGACGACGTATTGTGTTG
ATGCTGGCTGGTGGCGACAAGTCCAGCCAGCGCAGGGACATTGAGGCCGCCAAGGTGCTGGCCAGGGAATATGGCGATGG
CACCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT217622 WP_000554758.1 NZ_CP083869:c3698363-3698070 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217622 NZ_CP114542:c2880937-2880629 [Brevundimonas subvibrioides]
ATGGCACCTGATACCATTCCGTTCGATCCGGCGCGATATCTGAATGCCCCGGAAGCGATCGAGGAATACCTGGTCGCCTC
GTTCGAGGAGGCGGCCGAGTACGGCGATCCAGGAATCATCACCAAAGCCCTCGGCACGGTCGCCCGCGCCAAGGGCATGA
CGAAGATGGCAGAGGATGCCGAACTATCCCGGACAGCCCTTTACCGTGCGCTCAGCGCCGAGGGTCGTCCCGAACTGGCC
ACCATCTTCAAGGTGATGCAGGCCCTGGGCCTGCGCCTCGCGCCTGTACGCATGAAGCCTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3