Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 647133..647932 Replicon chromosome
Accession NZ_CP083869
Organism Escherichia coli strain NDM6

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag LCE24_RS04500 Protein ID WP_000347272.1
Coordinates 647133..647597 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCE24_RS04505 Protein ID WP_001307405.1
Coordinates 647597..647932 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE24_RS04470 (642134) 642134..642568 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCE24_RS04475 (642586) 642586..643464 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
LCE24_RS04480 (643454) 643454..644233 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCE24_RS04485 (644244) 644244..644717 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCE24_RS04490 (644740) 644740..646020 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCE24_RS04495 (646269) 646269..647078 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
LCE24_RS04500 (647133) 647133..647597 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCE24_RS04505 (647597) 647597..647932 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCE24_RS04510 (648081) 648081..649652 - 1572 WP_001273738.1 galactarate dehydratase -
LCE24_RS04515 (650027) 650027..651361 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LCE24_RS04520 (651377) 651377..652147 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T217606 WP_000347272.1 NZ_CP083869:c647597-647133 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T217606 NZ_CP114518:c5669-5418 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT217606 WP_001307405.1 NZ_CP083869:c647932-647597 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT217606 NZ_CP114518:c5928-5662 [Ligilactobacillus salivarius]
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References