Detailed information of TA system TA217602

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107542..107963
• Replicon: plasmid unnamed1
• Accession: NZ_CP083867
• Organism: Escherichia coli strain NDM6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE24_RS00630
• Protein ID: WP_001375150.1
• Coordinates: 107847..107963 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107542..107740 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE24_RS00590 (103238)	103238..104632	+	1395	WP_001037799.1	carbohydrate porin	-
LCE24_RS00595 (104878)	104878..104982	-	105	Protein_118	thiosulfate reductase cytochrome B subunit	-
LCE24_RS00600 (105039)	105039..105732	+	694	Protein_119	IS1 family transposase	-
LCE24_RS00605 (105859)	105859..106371	+	513	WP_223350513.1	potassium channel family protein	-
LCE24_RS00610 (106456)	106456..106707	+	252	Protein_121	iron-containing alcohol dehydrogenase	-
LCE24_RS00615 (106996)	106996..107313	-	318	Protein_122	IS110 family transposase	-
LCE24_RS00620 (107468)	107468..107573	+	106	Protein_123	plasmid SOS inhibition protein A	-
- (107542)	107542..107740	+	199	NuclAT_0	-	Antitoxin
- (107542)	107542..107740	+	199	NuclAT_0	-	Antitoxin
- (107542)	107542..107740	+	199	NuclAT_0	-	Antitoxin
- (107542)	107542..107740	+	199	NuclAT_0	-	Antitoxin
LCE24_RS00625 (107747)	107747..107896	+	150	Protein_124	DUF5431 family protein	-
LCE24_RS00630 (107847)	107847..107963	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE24_RS00635 (108264)	108264..108561	-	298	Protein_126	hypothetical protein	-
LCE24_RS00640 (108631)	108631..108837	+	207	WP_000275854.1	hypothetical protein	-
LCE24_RS00645 (108862)	108862..109149	+	288	WP_000107242.1	hypothetical protein	-
LCE24_RS00650 (109268)	109268..110089	+	822	WP_001234465.1	DUF932 domain-containing protein	-
LCE24_RS00655 (110385)	110385..110957	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
LCE24_RS00660 (111327)	111327..111710	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE24_RS00665 (111901)	111901..112548	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
LCE24_RS00670 (112684)	112684..112899	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / aadA2	-	1..224320	224320
	-	flank	IS/Tn	-	-	105355..105732	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T217602 WP_001375150.1 NZ_CP083867:107847-107963 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T217602 NZ_CP114511:123741-123992 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT217602 NZ_CP083867:107542-107740 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin