Detailed information of TA system TA217581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 63310..63956
• Replicon: chromosome
• Accession: NZ_CP083862
• Organism: Enterobacter kobei strain 11743-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD49_RS00325
• Protein ID: WP_032637796.1
• Coordinates: 63606..63956 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCD49_RS00320
• Protein ID: WP_032635284.1
• Coordinates: 63310..63609 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD49_RS00290 (LCD49_00295)	58723..59682	-	960	WP_023336511.1	DUF523 and DUF1722 domain-containing protein	-
LCD49_RS00295 (LCD49_00300)	59786..60196	-	411	WP_014881954.1	hydroxyisourate hydrolase	-
LCD49_RS00300 (LCD49_00305)	60296..61021	-	726	WP_045269330.1	MerR family transcriptional regulator	-
LCD49_RS00305 (LCD49_00310)	61139..61663	+	525	WP_045281336.1	lipocalin family protein	-
LCD49_RS00310 (LCD49_00315)	61742..62740	+	999	WP_045281334.1	class I SAM-dependent methyltransferase	-
LCD49_RS00315 (LCD49_00320)	62843..63280	+	438	WP_045281332.1	acetyltransferase	-
LCD49_RS00320 (LCD49_00325)	63310..63609	-	300	WP_032635284.1	XRE family transcriptional regulator	Antitoxin
LCD49_RS00325 (LCD49_00330)	63606..63956	-	351	WP_032637796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD49_RS00330 (LCD49_00335)	64219..64399	-	181	Protein_65	hydroxyacid dehydrogenase	-
LCD49_RS00335 (LCD49_00340)	64486..64827	-	342	WP_032635286.1	SymE family type I addiction module toxin	-
LCD49_RS00340 (LCD49_00345)	64958..65161	+	204	WP_071890797.1	toxin-antitoxin system HicB family antitoxin	-
LCD49_RS00345 (LCD49_00350)	65158..65343	-	186	WP_045281379.1	hypothetical protein	-
LCD49_RS00350 (LCD49_00355)	65345..65722	-	378	WP_208584490.1	hypothetical protein	-
LCD49_RS00360 (LCD49_00365)	66184..67575	+	1392	WP_045281331.1	glycoside-pentoside-hexuronide family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13483.49 Da
• Isoelectric Point: 6.9811

>T217581 WP_032637796.1 NZ_CP083862:c63956-63606 [Enterobacter kobei]
MWTVLFGKVFELWLLEQEEGLQEKVLADMLNLQNYGPRLPRPYADTVKGSQYNNMKELRVQYAGRPIRALFAFDPERQAI
VLCAGNKSNDKAFYEKLIRIADDEFTRHLTSLETAK

Nucleotide

• Length: 351 bp

>T217581 NZ_CP114375:733748-734233 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.04 Da
• Isoelectric Point: 6.9842

>AT217581 WP_032635284.1 NZ_CP083862:c63609-63310 [Enterobacter kobei]
VKTLNELIASRSPESQQRIKEMAEEMILETGLQLLREELRLSQKNVAELMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT217581 NZ_CP114375:733458-733751 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA