Detailed information of TA system TA217545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 80166..80830
• Replicon: chromosome
• Accession: NZ_CP083853
• Organism: Enterobacter roggenkampii strain 12795-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD45_RS00365
• Protein ID: WP_045337167.1
• Coordinates: 80462..80830 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B2S8A5
• Locus tag: LCD45_RS00360
• Protein ID: WP_045337168.1
• Coordinates: 80166..80465 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD45_RS00330 (LCD45_00335)	75568..76527	-	960	WP_032675710.1	DUF523 and DUF1722 domain-containing protein	-
LCD45_RS00335 (LCD45_00340)	76630..77040	-	411	WP_008500117.1	hydroxyisourate hydrolase	-
LCD45_RS00340 (LCD45_00345)	77140..77865	-	726	WP_008500116.1	MerR family transcriptional regulator	-
LCD45_RS00345 (LCD45_00350)	77983..78507	+	525	WP_008500115.1	lipocalin family protein	-
LCD45_RS00350 (LCD45_00355)	78586..79632	+	1047	WP_045337171.1	class I SAM-dependent methyltransferase	-
LCD45_RS00355 (LCD45_00360)	79699..80136	+	438	WP_045337169.1	acetyltransferase	-
LCD45_RS00360 (LCD45_00365)	80166..80465	-	300	WP_045337168.1	XRE family transcriptional regulator	Antitoxin
LCD45_RS00365 (LCD45_00370)	80462..80830	-	369	WP_045337167.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD45_RS00370 (LCD45_00375)	81261..81602	-	342	WP_045337166.1	SymE family type I addiction module toxin	-
LCD45_RS24580	81753..81881	+	129	WP_095442777.1	hypothetical protein	-
LCD45_RS00375 (LCD45_00380)	81887..82513	-	627	WP_052686642.1	hypothetical protein	-
LCD45_RS00385 (LCD45_00390)	82978..84369	+	1392	WP_058657501.1	glycoside-pentoside-hexuronide family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	67367..84369	17002

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14055.08 Da
• Isoelectric Point: 9.3784

>T217545 WP_045337167.1 NZ_CP083853:c80830-80462 [Enterobacter roggenkampii]
VDGSVRFGSVRFGKVFEQWLFEQEEGLQDKVLADLLNLQHYGPRLPRPYADTVKGSRYKHMKELRIQYAGRPVRAFFAFD
PVRQAIVLCAGDKSNDKTFYEKMIRIADAEFSLHLTSQEAAK

Nucleotide

• Length: 369 bp

>T217545 NZ_CP114354:c5136017-5135100 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCTAATCTGCAACAACAG
GCAGGTATAAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11121.06 Da
• Isoelectric Point: 6.9836

>AT217545 WP_045337168.1 NZ_CP083853:c80465-80166 [Enterobacter roggenkampii]
VKTLNEVIASRSPESQQRIKEMAEEMILETGLQLLREELMLSQKKVADLMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT217545 NZ_CP114354:c5136781-5136023 [Klebsiella variicola]
ATGTCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCAGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAACGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTAGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2S8A5