Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4369607..4370282 Replicon chromosome
Accession NZ_CP083849
Organism Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag LCD40_RS21185 Protein ID WP_015571638.1
Coordinates 4369983..4370282 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag LCD40_RS21180 Protein ID WP_015571639.1
Coordinates 4369607..4369972 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD40_RS21160 (LCD40_21185) 4366063..4366797 + 735 WP_017692800.1 class I SAM-dependent methyltransferase -
LCD40_RS21165 (LCD40_21190) 4366791..4367393 - 603 WP_015571642.1 short chain dehydrogenase -
LCD40_RS21170 (LCD40_21195) 4367491..4368378 + 888 WP_015571641.1 LysR family transcriptional regulator -
LCD40_RS21175 (LCD40_21200) 4368399..4369580 + 1182 WP_015571640.1 PLP-dependent aminotransferase family protein -
LCD40_RS21180 (LCD40_21205) 4369607..4369972 - 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
LCD40_RS21185 (LCD40_21210) 4369983..4370282 - 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCD40_RS21190 (LCD40_21215) 4370533..4371732 - 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
LCD40_RS21195 (LCD40_21220) 4371713..4373905 - 2193 WP_017692801.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T217542 WP_015571638.1 NZ_CP083849:c4370282-4369983 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T217542 NZ_CP114354:2040700-2040802 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT217542 WP_015571639.1 NZ_CP083849:c4369972-4369607 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT217542 NZ_CP114354:c2040808-2040664 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References