Detailed information of TA system TA217524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2917180..2917840
• Replicon: chromosome
• Accession: NZ_CP083842
• Organism: Enterobacter asburiae strain 16773-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD43_RS14065
• Protein ID: WP_100245677.1
• Coordinates: 2917487..2917840 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: LCD43_RS14060
• Protein ID: WP_029741460.1
• Coordinates: 2917180..2917482 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD43_RS14040 (LCD43_14050)	2913139..2915328	+	2190	WP_045403139.1	TonB-dependent siderophore receptor	-
LCD43_RS14050 (LCD43_14060)	2915652..2916041	-	390	WP_023312340.1	RidA family protein	-
LCD43_RS14055 (LCD43_14065)	2916183..2917103	+	921	WP_045403136.1	LysR family transcriptional regulator	-
LCD43_RS14060 (LCD43_14070)	2917180..2917482	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
LCD43_RS14065 (LCD43_14075)	2917487..2917840	-	354	WP_100245677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD43_RS14070 (LCD43_14080)	2918018..2918911	-	894	WP_047063523.1	LysR substrate-binding domain-containing protein	-
LCD43_RS14075 (LCD43_14085)	2919082..2919690	+	609	WP_057061635.1	glutathione S-transferase family protein	-
LCD43_RS14080 (LCD43_14090)	2919734..2920684	-	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
LCD43_RS14085 (LCD43_14095)	2920781..2921698	-	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13432.23 Da
• Isoelectric Point: 9.0310

>T217524 WP_100245677.1 NZ_CP083842:c2917840-2917487 [Enterobacter asburiae]
VSAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T217524 NZ_CP114332:795335-795745 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTCCCCTGGCCGCTGAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGCACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT217524 WP_029741460.1 NZ_CP083842:c2917482-2917180 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT217524 NZ_CP114332:795088-795354 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79