Detailed information of TA system TA217506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3718466..3719123
• Replicon: chromosome
• Accession: NZ_CP083834
• Organism: Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD48_RS18165
• Protein ID: WP_045899532.1
• Coordinates: 3718466..3718765 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A808ICP3
• Locus tag: LCD48_RS18170
• Protein ID: WP_033146339.1
• Coordinates: 3718776..3719123 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD48_RS18155 (LCD48_18155)	3714827..3717026	+	2200	Protein_3559	type I secretion system permease/ATPase	-
LCD48_RS18160 (LCD48_18160)	3717037..3718205	+	1169	Protein_3560	HlyD family efflux transporter periplasmic adaptor subunit	-
LCD48_RS18165 (LCD48_18165)	3718466..3718765	+	300	WP_045899532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD48_RS18170 (LCD48_18170)	3718776..3719123	+	348	WP_033146339.1	HigA family addiction module antitoxin	Antitoxin
LCD48_RS18175 (LCD48_18175)	3719168..3719581	+	414	WP_045888786.1	nucleoside triphosphatase NudI	-
LCD48_RS18180 (LCD48_18180)	3719553..3720284	-	732	WP_045888787.1	helix-turn-helix transcriptional regulator	-
LCD48_RS18185 (LCD48_18185)	3720382..3720801	+	420	WP_045888788.1	hypothetical protein	-
LCD48_RS18190 (LCD48_18190)	3720804..3721322	-	519	WP_045888789.1	hypothetical protein	-
LCD48_RS18195 (LCD48_18195)	3721407..3722588	-	1182	WP_045888790.1	PLP-dependent aminotransferase family protein	-
LCD48_RS18200 (LCD48_18200)	3722609..3723496	-	888	WP_033146345.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11641.26 Da
• Isoelectric Point: 10.0345

>T217506 WP_045899532.1 NZ_CP083834:3718466-3718765 [Enterobacter asburiae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNSQYRLVFRW
TEGKAEDLYLSPRKYTQHK

Nucleotide

• Length: 300 bp

>T217506 NZ_CP114328:301363-301638 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13066.02 Da
• Isoelectric Point: 7.0134

>AT217506 WP_033146339.1 NZ_CP083834:3718776-3719123 [Enterobacter asburiae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT217506 NZ_CP114328:301635-301994 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A808ICP3