Detailed information of TA system TA217504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3023206..3023866
• Replicon: chromosome
• Accession: NZ_CP083834
• Organism: Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD48_RS14825
• Protein ID: WP_039263808.1
• Coordinates: 3023513..3023866 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: LCD48_RS14820
• Protein ID: WP_029741460.1
• Coordinates: 3023206..3023508 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD48_RS14800 (LCD48_14795)	3019060..3021249	+	2190	WP_045142987.1	TonB-dependent siderophore receptor	-
LCD48_RS14810 (LCD48_14805)	3021679..3022068	-	390	WP_045142988.1	RidA family protein	-
LCD48_RS14815 (LCD48_14810)	3022210..3023130	+	921	WP_039263806.1	LysR family transcriptional regulator	-
LCD48_RS14820 (LCD48_14815)	3023206..3023508	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
LCD48_RS14825 (LCD48_14820)	3023513..3023866	-	354	WP_039263808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD48_RS14830 (LCD48_14825)	3024044..3024937	-	894	WP_039263809.1	LysR substrate-binding domain-containing protein	-
LCD48_RS14835 (LCD48_14830)	3025111..3025716	+	606	WP_045887896.1	glutathione S-transferase family protein	-
LCD48_RS14840 (LCD48_14835)	3025760..3026710	-	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
LCD48_RS14845 (LCD48_14840)	3026807..3027724	-	918	WP_045887835.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13586.44 Da
• Isoelectric Point: 9.7160

>T217504 WP_039263808.1 NZ_CP083834:c3023866-3023513 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNRLKERE

Nucleotide

• Length: 354 bp

>T217504 NZ_CP114325:67656-68138 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT217504 WP_029741460.1 NZ_CP083834:c3023508-3023206 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT217504 NZ_CP114325:67402-67668 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79