Detailed information of TA system TA217476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1781739..1782399
• Replicon: chromosome
• Accession: NZ_CP083830
• Organism: Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD44_RS08700
• Protein ID: WP_100245677.1
• Coordinates: 1781739..1782092 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: LCD44_RS08705
• Protein ID: WP_029741460.1
• Coordinates: 1782097..1782399 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD44_RS08680 (LCD44_08700)	1777881..1778798	+	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-
LCD44_RS08685 (LCD44_08705)	1778895..1779845	+	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
LCD44_RS08690 (LCD44_08710)	1779889..1780497	-	609	WP_057061635.1	glutathione S-transferase family protein	-
LCD44_RS08695 (LCD44_08715)	1780668..1781561	+	894	WP_047063523.1	LysR substrate-binding domain-containing protein	-
LCD44_RS08700 (LCD44_08720)	1781739..1782092	+	354	WP_100245677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD44_RS08705 (LCD44_08725)	1782097..1782399	+	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
LCD44_RS08710 (LCD44_08730)	1782476..1783396	-	921	WP_045403136.1	LysR family transcriptional regulator	-
LCD44_RS08715 (LCD44_08735)	1783538..1783927	+	390	WP_023312340.1	RidA family protein	-
LCD44_RS08725 (LCD44_08745)	1784251..1786440	-	2190	WP_045403139.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13432.23 Da
• Isoelectric Point: 9.0310

>T217476 WP_100245677.1 NZ_CP083830:1781739-1782092 [Enterobacter asburiae]
VSAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T217476 NZ_CP114320:301363-301638 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT217476 WP_029741460.1 NZ_CP083830:1782097-1782399 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT217476 NZ_CP114320:301635-301994 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79