Detailed information of TA system TA217460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4669472..4670074
• Replicon: chromosome
• Accession: NZ_CP083821
• Organism: Enterobacter cloacae subsp. cloacae strain 12961-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: LCD47_RS22385
• Protein ID: WP_013099360.1
• Coordinates: 4669763..4670074 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCD47_RS22380
• Protein ID: WP_013099359.1
• Coordinates: 4669472..4669762 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD47_RS22365 (LCD47_22395)	4666969..4667871	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
LCD47_RS22370 (LCD47_22400)	4667868..4668503	+	636	WP_023622447.1	formate dehydrogenase cytochrome b556 subunit	-
LCD47_RS22375 (LCD47_22405)	4668500..4669429	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
LCD47_RS22380 (LCD47_22410)	4669472..4669762	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
LCD47_RS22385 (LCD47_22415)	4669763..4670074	-	312	WP_013099360.1	hypothetical protein	Toxin
LCD47_RS22390 (LCD47_22420)	4670304..4670429	+	126	Protein_4365	alpha/beta hydrolase	-
LCD47_RS22395 (LCD47_22425)	4670508..4671449	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
LCD47_RS22400 (LCD47_22430)	4671494..4671931	-	438	WP_013099363.1	D-aminoacyl-tRNA deacylase	-
LCD47_RS22405 (LCD47_22435)	4671928..4672809	-	882	WP_013099364.1	virulence factor BrkB family protein	-
LCD47_RS22410 (LCD47_22440)	4672803..4673402	-	600	WP_139937197.1	glucose-1-phosphatase	-
LCD47_RS22415 (LCD47_22445)	4673522..4674322	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T217460 WP_013099360.1 NZ_CP083821:c4670074-4669763 [Enterobacter cloacae subsp. cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T217460 NZ_CP114314:c14256-13906 [Klebsiella variicola]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT217460 WP_013099359.1 NZ_CP083821:c4669762-4669472 [Enterobacter cloacae subsp. cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT217460 NZ_CP114314:c13901-13599 [Klebsiella variicola]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02