Detailed information of TA system TA217446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1166463..1167112
• Replicon: chromosome
• Accession: NZ_CP083821
• Organism: Enterobacter cloacae subsp. cloacae strain 12961-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD47_RS05535
• Protein ID: WP_038981089.1
• Coordinates: 1166750..1167112 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5E1A3P3
• Locus tag: LCD47_RS05530
• Protein ID: WP_013097693.1
• Coordinates: 1166463..1166762 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD47_RS05515 (LCD47_05525)	1161587..1163560	+	1974	WP_045902386.1	PhoX family phosphatase	-
LCD47_RS05520 (LCD47_05530)	1163663..1165051	+	1389	WP_139937276.1	phenylalanine transporter	-
LCD47_RS05525 (LCD47_05535)	1165242..1166333	+	1092	WP_226842839.1	IS4 family transposase	-
LCD47_RS05530 (LCD47_05540)	1166463..1166762	-	300	WP_013097693.1	helix-turn-helix transcriptional regulator	Antitoxin
LCD47_RS05535 (LCD47_05545)	1166750..1167112	-	363	WP_038981089.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD47_RS05540 (LCD47_05550)	1167317..1168555	+	1239	WP_139937286.1	MFS transporter	-
LCD47_RS05545 (LCD47_05555)	1168571..1169596	+	1026	WP_191979764.1	sugar phosphate isomerase/epimerase	-
LCD47_RS05550 (LCD47_05560)	1169589..1170731	+	1143	WP_058651679.1	Gfo/Idh/MocA family oxidoreductase	-
LCD47_RS05555 (LCD47_05565)	1170807..1171778	+	972	WP_038418845.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14022.02 Da
• Isoelectric Point: 7.3237

>T217446 WP_038981089.1 NZ_CP083821:c1167112-1166750 [Enterobacter cloacae subsp. cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYREMIALADVEYHRHLANKEAIWQR

Nucleotide

• Length: 363 bp

>T217446 NZ_CP114312:301363-301638 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10875.62 Da
• Isoelectric Point: 8.6152

>AT217446 WP_013097693.1 NZ_CP083821:c1166762-1166463 [Enterobacter cloacae subsp. cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Nucleotide

• Length: 300 bp

>AT217446 NZ_CP114312:301635-301994 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1A3P3