Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1093797..1094446 Replicon chromosome
Accession NZ_CP083819
Organism Enterobacter roggenkampii strain 13840-yvys

Toxin (Protein)


Gene name higB Uniprot ID A0A1S2AEP5
Locus tag LCD41_RS05210 Protein ID WP_045416590.1
Coordinates 1094084..1094446 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1S2AD04
Locus tag LCD41_RS05205 Protein ID WP_047746098.1
Coordinates 1093797..1094096 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD41_RS05195 (LCD41_05210) 1090221..1092194 + 1974 WP_047746094.1 PhoX family phosphatase -
LCD41_RS05200 (LCD41_05215) 1092298..1093686 + 1389 WP_047746095.1 phenylalanine transporter -
LCD41_RS05205 (LCD41_05220) 1093797..1094096 - 300 WP_047746098.1 helix-turn-helix transcriptional regulator Antitoxin
LCD41_RS05210 (LCD41_05225) 1094084..1094446 - 363 WP_045416590.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCD41_RS05215 (LCD41_05230) 1094652..1095887 + 1236 WP_032663994.1 MFS transporter -
LCD41_RS05220 (LCD41_05235) 1095903..1096928 + 1026 WP_047746100.1 sugar phosphate isomerase/epimerase -
LCD41_RS05225 (LCD41_05240) 1096921..1098063 + 1143 WP_047746102.1 Gfo/Idh/MocA family oxidoreductase -
LCD41_RS05230 (LCD41_05245) 1098139..1099110 + 972 WP_021241589.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1082791..1094446 11655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-87)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13908.76 Da        Isoelectric Point: 5.1960

>T217429 WP_045416590.1 NZ_CP083819:c1094446-1094084 [Enterobacter roggenkampii]
MWNVETTDRFDDWFFEQTTALKEDVLATMHILAEFGPTLGRPYVDTVKDSAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMIALADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T217429 NZ_CP114305:c61177-60869 [Klebsiella oxytoca]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CGAGCGCAATACTCGGGTTGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACTCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAG
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10906.70 Da        Isoelectric Point: 9.8627

>AT217429 WP_047746098.1 NZ_CP083819:c1094096-1093797 [Enterobacter roggenkampii]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTLAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT217429 NZ_CP114305:c61416-61180 [Klebsiella oxytoca]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCTGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCTGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2AEP5

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2AD04

References