Detailed information of TA system TA217402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2416398..2417125
• Replicon: chromosome
• Accession: NZ_CP083809
• Organism: Pantoea agglomerans strain 33.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LB453_RS14210
• Protein ID: WP_103795498.1
• Coordinates: 2416398..2416709 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LB453_RS14215
• Protein ID: WP_103795497.1
• Coordinates: 2416706..2417125 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB453_RS14180 (LB453_14140)	2411449..2412204	+	756	WP_224481450.1	glycosyltransferase family 25 protein	-
LB453_RS14185 (LB453_14145)	2412218..2412834	-	617	Protein_2296	tail fiber assembly protein	-
LB453_RS14190 (LB453_14150)	2412834..2413369	-	536	Protein_2297	phage tail protein	-
LB453_RS14195 (LB453_14155)	2413410..2413796	+	387	Protein_2298	tyrosine-type recombinase/integrase	-
LB453_RS14200 (LB453_14160)	2413985..2414758	+	774	WP_103795501.1	spermidine/putrescine ABC transporter permease PotC	-
LB453_RS14205 (LB453_14165)	2414769..2415812	+	1044	WP_103795500.1	spermidine/putrescine ABC transporter substrate-binding protein PotD	-
LB453_RS14210 (LB453_14170)	2416398..2416709	+	312	WP_103795498.1	type II toxin-antitoxin system HigB family toxin	Toxin
LB453_RS14215 (LB453_14175)	2416706..2417125	+	420	WP_103795497.1	helix-turn-helix domain-containing protein	Antitoxin
LB453_RS14220 (LB453_14180)	2417319..2417579	+	261	Protein_2303	maturation control protein	-
LB453_RS14225 (LB453_14185)	2417799..2418632	-	834	WP_103795496.1	NAD-dependent protein deacylase	-
LB453_RS14230 (LB453_14190)	2418725..2419969	-	1245	WP_103795495.1	lipoprotein-releasing ABC transporter permease subunit LolE	-
LB453_RS14235 (LB453_14195)	2419969..2420673	-	705	WP_103795494.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
LB453_RS14240 (LB453_14200)	2420666..2421865	-	1200	WP_103795493.1	lipoprotein-releasing ABC transporter permease subunit LolC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12403.21 Da
• Isoelectric Point: 9.9437

>T217402 WP_103795498.1 NZ_CP083809:2416398-2416709 [Pantoea agglomerans]
MHVISKEPFEQAARLFPNDARAIKALYRLVRETDFKTPEELRAAVPSLDHFKYRDKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T217402 NZ_CP114266:12127-12390 [Acinetobacter sp. TR3]
ATGCATGTATTTACATATACGGACGCACGTAACAATTTAAAATCTGTTTTAGATAAAGTAATTGATGATGTAGACGTTGC
TTTTATTACAAGAAAAGAAGGTGGCCATGCTGTAGTAATGGGACAAGATCATTATGATAGCTTAATGGAAACGTTATATC
TCCTTTCATCTCCAAATAATGCAAGTCGTTTAAATGAATCAATTGCTCAATTACGTGCAGGGAAAACAAAACACAGAGAG
TTGATTGAGGATGACGAATCGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15472.57 Da
• Isoelectric Point: 4.6545

>AT217402 WP_103795497.1 NZ_CP083809:2416706-2417125 [Pantoea agglomerans]
MIADTIKAIEATKQLVAAVPLLGGSNSEKDYRDALALVDYLIDHDDENPLIDFLAAKIAHYEDNSERFAEFNKEVDEMPV
GVALLRTLIDQHRLSYSDLSEEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPQWFL

Nucleotide

• Length: 420 bp

>AT217402 NZ_CP114266:12377-12640 [Acinetobacter sp. TR3]
ATGACGAATCGTAACGTCGAATGGACTGATAATGCCTGGGATGAATATATCTATTGGCAGACACAGGATAAAAAGATACT
TAAGCGTATTAATACCTTAATCAAAGAATGTCAGCGAACACCTTTTGAAGGAACAGGAAAACCAGAACCTTTAAAAGCTA
ATCTTTCAGGATTTTGGAGTCGTAGGATTGATGAAAAGCATAGATTAGTTTATGAAGTGACAGATGAACGAATCTCTATA
ATTCAATGTCGATTCCATTACTAA