Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 357648..358294 Replicon chromosome
Accession NZ_CP083796
Organism Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag LB481_RS01655 Protein ID WP_016529833.1
Coordinates 357648..357995 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LB481_RS01660 Protein ID WP_002920557.1
Coordinates 357995..358294 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB481_RS01645 (353574) 353574..355007 + 1434 WP_002920564.1 glycogen synthase GlgA -
LB481_RS01650 (355025) 355025..357472 + 2448 WP_002920561.1 glycogen phosphorylase -
LB481_RS01655 (357648) 357648..357995 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LB481_RS01660 (357995) 357995..358294 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LB481_RS01665 (358357) 358357..359865 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
LB481_RS01670 (360070) 360070..360399 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LB481_RS01675 (360450) 360450..361280 + 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
LB481_RS01680 (361330) 361330..362088 + 759 WP_032456686.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T217357 WP_016529833.1 NZ_CP083796:357648-357995 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T217357 NZ_CP114166:c14256-13906 [Klebsiella variicola]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT217357 WP_002920557.1 NZ_CP083796:357995-358294 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT217357 NZ_CP114166:c13901-13599 [Klebsiella variicola]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References