Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 345403..346049 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag LB484_RS01575 Protein ID WP_008806991.1
Coordinates 345403..345750 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag LB484_RS01580 Protein ID WP_008806992.1
Coordinates 345750..346049 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS01565 (LB484_01565) 341290..342723 + 1434 WP_004202133.1 glycogen synthase GlgA -
LB484_RS01570 (LB484_01570) 342741..345188 + 2448 WP_008806990.1 glycogen phosphorylase -
LB484_RS01575 (LB484_01575) 345403..345750 + 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LB484_RS01580 (LB484_01580) 345750..346049 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
LB484_RS01585 (LB484_01585) 346112..347620 - 1509 WP_032740278.1 glycerol-3-phosphate dehydrogenase -
LB484_RS01590 (LB484_01590) 347825..348154 + 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
LB484_RS01595 (LB484_01595) 348205..349035 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
LB484_RS01600 (LB484_01600) 349085..349843 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T217336 WP_008806991.1 NZ_CP083790:345403-345750 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T217336 NZ_CP114160:c2720787-2719465 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGCTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGTTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGTATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTCTGCTG
CCCGGCGGCAGCTATCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCGGCAAGGGCTGCAGGCGCGATAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT217336 WP_008806992.1 NZ_CP083790:345750-346049 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT217336 NZ_CP114160:c2721053-2720787 [Klebsiella quasipneumoniae]
ATGCATTCCCCGCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CCACCCTGTTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAGCCGGCCGAGACGGCGACGGCCCCC
GATAACACTACGGATCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References