Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 1374..2004 Replicon plasmid pKSB2_6C_1
Accession NZ_CP083785
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name higB Uniprot ID A0A3V9PY09
Locus tag LD127_RS25650 Protein ID WP_001708474.1
Coordinates 1669..2004 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A704FLZ1
Locus tag LD127_RS25645 Protein ID WP_001708475.1
Coordinates 1374..1664 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS25640 661..1314 - 654 WP_001708476.1 hypothetical protein -
LD127_RS25645 1374..1664 - 291 WP_001708475.1 NadS family protein Antitoxin
LD127_RS25650 1669..2004 - 336 WP_001708474.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LD127_RS25655 2209..2877 - 669 WP_044296050.1 hypothetical protein -
LD127_RS25660 2927..3253 - 327 WP_044296048.1 hypothetical protein -
LD127_RS25665 3566..4126 - 561 WP_052957587.1 hypothetical protein -
LD127_RS25670 4144..4494 - 351 WP_142762106.1 hypothetical protein -
LD127_RS25675 4529..4864 - 336 WP_094340933.1 hypothetical protein -
LD127_RS25680 4861..5130 - 270 WP_024172976.1 hypothetical protein -
LD127_RS25685 5144..5521 - 378 WP_069346704.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..44303 44303


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-104)

Antitoxin

Antitoxin MqsA/HigA-2

(21-94)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12615.30 Da        Isoelectric Point: 5.2407

>T217333 WP_001708474.1 NZ_CP083785:c2004-1669 [Klebsiella pneumoniae]
MEYYEFVETSVFTREMKTLLSDDEYKEFQTFLIENPEAGDLIVGTGGCRKVRWSRQGTGKSSGVRAIYYFYNPAGRLYML
IIYPKSEKDSLTAAEKNQLKAVVAGFKGEEG

Download         Length: 336 bp

>T217333 NZ_CP114160:812921-813331 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10909.62 Da        Isoelectric Point: 10.0073

>AT217333 WP_001708475.1 NZ_CP083785:c1664-1374 [Klebsiella pneumoniae]
MRKELFDDLIASAKEAVEIHQGLREPARVTRFERPDVKAIRAKVHMKQDDFASLIGVSSSLVQSWEQGLRTPAGAALKLL
RVIERKPEFVQELREA

Download         Length: 291 bp

>AT217333 NZ_CP114160:812674-812940 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V9PY09

Antitoxin

Source ID Structure
AlphaFold DB A0A704FLZ1

References