Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 362096..362742 Replicon chromosome
Accession NZ_CP083784
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name higB Uniprot ID A0A483JFR4
Locus tag LD127_RS01675 Protein ID WP_004188313.1
Coordinates 362096..362443 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A483GM64
Locus tag LD127_RS01680 Protein ID WP_004188315.1
Coordinates 362443..362742 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS01665 (358022) 358022..359455 + 1434 WP_002920564.1 glycogen synthase GlgA -
LD127_RS01670 (359473) 359473..361920 + 2448 WP_002920561.1 glycogen phosphorylase -
LD127_RS01675 (362096) 362096..362443 + 348 WP_004188313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LD127_RS01680 (362443) 362443..362742 + 300 WP_004188315.1 XRE family transcriptional regulator Antitoxin
LD127_RS01685 (362805) 362805..364313 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LD127_RS01690 (364518) 364518..364847 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LD127_RS01695 (364898) 364898..365728 + 831 WP_004188317.1 rhomboid family intramembrane serine protease GlpG -
LD127_RS01700 (365778) 365778..366536 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-78)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13548.57 Da        Isoelectric Point: 6.2327

>T217315 WP_004188313.1 NZ_CP083784:362096-362443 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T217315 NZ_CP114149:c4355946-4355659 [Klebsiella michiganensis]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10873.64 Da        Isoelectric Point: 10.1464

>AT217315 WP_004188315.1 NZ_CP083784:362443-362742 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAAWG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT217315 NZ_CP114149:c4356185-4355946 [Klebsiella michiganensis]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483JFR4

Antitoxin

Source ID Structure
AlphaFold DB A0A483GM64

References