Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3797350..3797947 Replicon chromosome
Accession NZ_CP083768
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LB480_RS18700 Protein ID WP_004142563.1
Coordinates 3797630..3797947 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LB480_RS18695 Protein ID WP_004142561.1
Coordinates 3797350..3797637 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS18665 (3793430) 3793430..3793678 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LB480_RS18670 (3793696) 3793696..3794037 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LB480_RS18675 (3794068) 3794068..3795183 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LB480_RS18680 (3795363) 3795363..3795944 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LB480_RS18685 (3795944) 3795944..3796312 + 369 WP_040236580.1 MmcQ/YjbR family DNA-binding protein -
LB480_RS18690 (3796432) 3796432..3797085 + 654 WP_004176973.1 oxygen-insensitive NAD(P)H nitroreductase -
LB480_RS18695 (3797350) 3797350..3797637 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LB480_RS18700 (3797630) 3797630..3797947 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LB480_RS18705 (3798132) 3798132..3799175 - 1044 WP_040236579.1 DUF2157 domain-containing protein -
LB480_RS18710 (3799845) 3799845..3800711 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LB480_RS18715 (3800820) 3800820..3802247 + 1428 WP_040236578.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T217281 WP_004142563.1 NZ_CP083768:c3797947-3797630 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T217281 NZ_CP114130:1036380-1036715 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT217281 WP_004142561.1 NZ_CP083768:c3797637-3797350 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT217281 NZ_CP114130:1036132-1036380 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References