Detailed information of TA system TA217253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1857126..1857786
• Replicon: chromosome
• Accession: NZ_CP083754
• Organism: Serratia marcescens strain YL4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A656VMD8
• Locus tag: LBP97_RS08810
• Protein ID: WP_025303493.1
• Coordinates: 1857433..1857786 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8G2G5Z5
• Locus tag: LBP97_RS08805
• Protein ID: WP_016927038.1
• Coordinates: 1857126..1857428 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBP97_RS08785 (LBP97_08795)	1852730..1853644	-	915	WP_004928592.1	branched-chain amino acid ABC transporter permease	-
LBP97_RS08790 (LBP97_08800)	1853770..1854888	-	1119	WP_016927040.1	branched-chain amino acid ABC transporter substrate-binding protein	-
LBP97_RS08800 (LBP97_08810)	1855494..1856702	-	1209	WP_238969517.1	aldose 1-epimerase family protein	-
LBP97_RS08805 (LBP97_08815)	1857126..1857428	-	303	WP_016927038.1	XRE family transcriptional regulator	Antitoxin
LBP97_RS08810 (LBP97_08820)	1857433..1857786	-	354	WP_025303493.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LBP97_RS08815 (LBP97_08825)	1858225..1859520	+	1296	WP_025303494.1	MFS transporter	-
LBP97_RS08820 (LBP97_08830)	1859547..1860353	+	807	WP_060447908.1	substrate-binding domain-containing protein	-
LBP97_RS08825 (LBP97_08835)	1860328..1861227	-	900	WP_043143085.1	LysR family transcriptional regulator	-
LBP97_RS08830 (LBP97_08840)	1861331..1861696	-	366	WP_004935528.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13548.47 Da
• Isoelectric Point: 9.0170

>T217253 WP_025303493.1 NZ_CP083754:c1857786-1857433 [Serratia marcescens]
VWEIKTTDAFDHWYRSLSDADRAGVLAALMVLREKGPGLPRPYADTVKGSRYSNMKELRIQCRGEPLRAFFAFDPYRIGI
VLCAGNKAGNEKRFYDRMIQVADREFTNWLNTLNEKE

Nucleotide

• Length: 354 bp

>T217253 NZ_CP114115:c6638077-6637790 [Pseudomonas sp. GXZC]
ATGACCTATGAACTCGACTTCGATGCGCGCGCTTTAAAGGAATGGCACAAACTTGGCGATACCGTTCGCCAACAGTTTAA
AAAGAAGTTGGCCGCAGTCTTGATCAACCCACGTATCGAAGCCAGCCGTCTGTACGGCTTACCCGATTGCTACAAGATCA
AGCTTCGCAGCAGTGGCTACCGGTTGGTGTATCAAGTAATTGATCAGGAGATCACGGTATTTGTCGTTGCTGTCGACAAG
CGTGAACGTGAAGAGGTCTATCGGAAGGCTGCCGACCGGTTGAGCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11093.96 Da
• Isoelectric Point: 6.4738

>AT217253 WP_016927038.1 NZ_CP083754:c1857428-1857126 [Serratia marcescens]
MGRTLEQLIADEKPEVVAKAQALAADIMLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDIELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT217253 NZ_CP114115:c6638306-6638067 [Pseudomonas sp. GXZC]
ATGGCCTCTATTAACGTACGTATCGACGACGATCTTAAGGCTCGTGCTTATCACGAGCTGGAAAAACTCGGCGTCACCCC
ATCGGAGTTAATGCGCCAAGCTTTGCAGTACGTGGCAGAGCGCGGTCAGTTACCCTTCAAACCTGTGCTCATGACTGAAG
ATGACGAGGCTTTGTTGGCAACTGTTCGGGAGCGGTTGGCTGCGCCTCAACGAGTGAAGGTCTCATTGGATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A656VMD8