Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 327133..327719 Replicon chromosome
Accession NZ_CP083751
Organism Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LBE00_RS01530 Protein ID WP_002920800.1
Coordinates 327351..327719 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LBE00_RS01525 Protein ID WP_004174006.1
Coordinates 327133..327354 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBE00_RS01505 (LBE00_01505) 323290..324216 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LBE00_RS01510 (LBE00_01510) 324213..325490 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LBE00_RS01515 (LBE00_01515) 325487..326254 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LBE00_RS01520 (LBE00_01520) 326256..326969 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LBE00_RS01525 (LBE00_01525) 327133..327354 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LBE00_RS01530 (LBE00_01530) 327351..327719 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LBE00_RS01535 (LBE00_01535) 327992..329308 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LBE00_RS01540 (LBE00_01540) 329415..330302 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LBE00_RS01545 (LBE00_01545) 330299..331144 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LBE00_RS01550 (LBE00_01550) 331146..332216 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 311743..332953 21210


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T217223 WP_002920800.1 NZ_CP083751:327351-327719 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T217223 NZ_CP114058:328293-328469 [Rouxiella chamberiensis]
GTGAAGCAAAGCGAGTTCAGGCGGTGGCTTGAATCTCAAGGGGTAGAAGTTTACGACGGTACCAAACATCTAAAACTTCG
ATTCAAAGGCAAGCGTAGTGTGATGCCAAGACACCCCGGCTCTGAGTTAGATGACCGACTCAGAAAAGTGATTCTCAAGC
AATTAGGCCTTAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT217223 WP_004174006.1 NZ_CP083751:327133-327354 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT217223 NZ_CP114058:328519-328926 [Rouxiella chamberiensis]
ATGCGATACCCAGTAACCTTAGAACCCGATAACGGCGTATACCTTGTGCAGTTTCCCGACATCCCCGAAGCTTTAACGCA
GGGAGGATCACGGGAAGAAGCCTTGGAAATGGCGCTCGATGCTTTGGTAACATCCTTTGAGTTTTACTTTGAGGATAGTG
AAAAGGTTCCAGCGCCAAGCGCCGTGACAGGTGATTATGTTGAGGTGCCTGCCAGCGTGACAGCCAAAGTGATAATGCTG
AACGCTTTCATTGATTCAGGATTGACGCAAATTCAGTTGGCGAACGCGATGGGCGTGAAAAAGCAGGAAGTGACCAGGTT
GTTTGACCTGCAGCATTCCACCAAGATTGACACTATCCAAAAGGCTCTTTCGGCCTTGGGAAAGCAACTTGAACTTGTCG
CTGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References