Detailed information of TA system TA021721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5514196..5514791
• Replicon: chromosome
• Accession: NC_008463
• Organism: Pseudomonas aeruginosa UCBPP-PA14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA14_RS30995
• Protein ID: WP_003113526.1
• Coordinates: 5514513..5514791 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA14_RS25265
• Protein ID: WP_003133769.1
• Coordinates: 5514196..5514501 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA14_RS25230 (PA14_61750)	5509336..5510184	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PA14_RS25240 (PA14_61770)	5510351..5511292	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
PA14_RS25245 (PA14_61780)	5511409..5512023	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
PA14_RS25250 (PA14_61790)	5512065..5512649	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PA14_RS25255 (PA14_61820)	5512690..5513790	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
PA14_RS25265 (PA14_61840)	5514196..5514501	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
PA14_RS30995	5514513..5514791	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA14_RS25270 (PA14_61850)	5515120..5517348	+	2229	WP_003141628.1	TonB-dependent receptor	-
PA14_RS25275 (PA14_61860)	5517418..5518065	-	648	WP_003095021.1	carbonate dehydratase	-
PA14_RS25280 (PA14_61870)	5518127..5519365	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T21721 WP_003113526.1 NC_008463:c5514791-5514513 [Pseudomonas aeruginosa UCBPP-PA14]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T21721 NC_008463:c5514791-5514513 [Pseudomonas aeruginosa UCBPP-PA14]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT21721 WP_003133769.1 NC_008463:c5514501-5514196 [Pseudomonas aeruginosa UCBPP-PA14]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT21721 NC_008463:c5514501-5514196 [Pseudomonas aeruginosa UCBPP-PA14]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3