Detailed information of TA system TA217197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4660239..4660993
• Replicon: chromosome
• Accession: NZ_CP083731
• Organism: Salmonella sp. A29-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: K8W53_RS22610
• Protein ID: WP_000558166.1
• Coordinates: 4660239..4660550 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8W53_RS22615
• Protein ID: WP_001259011.1
• Coordinates: 4660547..4660993 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8W53_RS22580 (4655897)	4655897..4656799	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
K8W53_RS22585 (4656796)	4656796..4657431	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K8W53_RS22590 (4657428)	4657428..4658357	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
K8W53_RS22595 (4658404)	4658404..4658694	-	291	WP_000362052.1	DNA-binding transcriptional regulator	-
K8W53_RS22600 (4658695)	4658695..4659006	-	312	WP_023138876.1	hypothetical protein	-
K8W53_RS22605 (4659224)	4659224..4660153	+	930	WP_001127698.1	alpha/beta hydrolase	-
K8W53_RS22610 (4660239)	4660239..4660550	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
K8W53_RS22615 (4660547)	4660547..4660993	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
K8W53_RS22620 (4661008)	4661008..4661949	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K8W53_RS22625 (4661994)	4661994..4662431	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
K8W53_RS22630 (4662428)	4662428..4663300	-	873	WP_000921423.1	virulence factor BrkB family protein	-
K8W53_RS22635 (4663294)	4663294..4663893	-	600	WP_000965695.1	glucose-1-phosphatase	-
K8W53_RS22640 (4664084)	4664084..4664887	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
K8W53_RS22645 (4664921)	4664921..4665817	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T217197 WP_000558166.1 NZ_CP083731:4660239-4660550 [Salmonella sp. A29-2]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T217197 NZ_CP113974:c5185490-5185209 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGTAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGAAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT217197 WP_001259011.1 NZ_CP083731:4660547-4660993 [Salmonella sp. A29-2]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT217197 NZ_CP113974:c5185714-5185487 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCGGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90