Detailed information of TA system TA217169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2416233..2416750
• Replicon: chromosome
• Accession: NZ_CP083728
• Organism: Staphylococcus aureus strain MR254

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: K9B11_RS11990
• Protein ID: WP_000113262.1
• Coordinates: 2416484..2416750 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: K9B11_RS11985
• Protein ID: WP_000584499.1
• Coordinates: 2416233..2416484 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9B11_RS11955 (2411931)	2411931..2412650	+	720	WP_000479576.1	amino acid ABC transporter permease	-
K9B11_RS11960 (2412647)	2412647..2413378	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
K9B11_RS11965 (2413475)	2413475..2414725	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
K9B11_RS11970 (2414980)	2414980..2415342	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
K9B11_RS11975 (2415361)	2415361..2415960	+	600	WP_000162813.1	DsbA family protein	-
K9B11_RS11980 (2415967)	2415967..2416068	+	102	WP_224433947.1	hypothetical protein	-
K9B11_RS11985 (2416233)	2416233..2416484	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K9B11_RS11990 (2416484)	2416484..2416750	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
K9B11_RS11995 (2416785)	2416785..2416856	+	72	Protein_2332	hypothetical protein	-
K9B11_RS12000 (2417045)	2417045..2418592	+	1548	WP_001551849.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
K9B11_RS12005 (2418778)	2418778..2419245	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
K9B11_RS12010 (2419329)	2419329..2419511	+	183	WP_000230294.1	hypothetical protein	-
K9B11_RS12015 (2419728)	2419728..2420552	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
K9B11_RS12020 (2420785)	2420785..2421321	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T217169 WP_000113262.1 NZ_CP083728:2416484-2416750 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T217169 NZ_CP113942:144460-144723 [Bifidobacterium sp. ESL0682]
GTGACCTATCGCGTTGTCATCAAAAATTCGGCTAAAGCGGATCTTAAAAAACTAAAGCGTTCACATCTTCAGCAGTCATT
TATGGAAGTTGTTGCGGATTTAAAATCCGATCCCTATCAGCCGACTCAATCATTCGAGAAGCTTCAGCCAACCAGTCGAG
GGTTGTATTCGCGCAGGATTAACAGTCAGCATCGTGTGGTCTACGATATCAATGACGATGCGCAGACGGTAACGATTTAT
GCAGCCTGGAGTCATTACGAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT217169 WP_000584499.1 NZ_CP083728:2416233-2416484 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT217169 NZ_CP113942:144200-144463 [Bifidobacterium sp. ESL0682]
ATGGAAACATATACACCCACGGCCGCGAGGAAGAATCTGTATCAGATCCTCAAAGACGTCAACATCCAACGAGAGCCTAT
CGTCATTTCTCCGGCAAGGGGCAACAAGGATGAAGAAGCCGTAGTTGTAAACCGCGCTGATTGGAATTCGATTATGGAAA
CGTTATATCTGGAGAATACCGGTACGCTTGCCACTGCTGAAGAACGTCGCAACGACAACAGCGGAATCACGGATATTGAT
GATATCGATTGGGATACGCTGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60