Detailed information of TA system TA217156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2351998..2352639
• Replicon: chromosome
• Accession: NZ_CP083718
• Organism: Cupriavidus metallidurans strain ZM02

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q1LE01
• Locus tag: LAI70_RS10395
• Protein ID: WP_011519196.1
• Coordinates: 2351998..2352282 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LAI70_RS10400
• Protein ID: WP_011519197.1
• Coordinates: 2352292..2352639 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAI70_RS10365 (LAI70_10365)	2347000..2347401	+	402	WP_224444192.1	RT0821/Lpp0805 family surface protein	-
LAI70_RS10370 (LAI70_10370)	2347497..2348087	-	591	WP_011519191.1	hypothetical protein	-
LAI70_RS10375 (LAI70_10375)	2348210..2348896	-	687	WP_029306340.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LAI70_RS10380 (LAI70_10380)	2349249..2350139	-	891	WP_224444193.1	helix-turn-helix transcriptional regulator	-
LAI70_RS10385 (LAI70_10385)	2350450..2350668	+	219	WP_008651241.1	YdcH family protein	-
LAI70_RS10390 (LAI70_10390)	2350670..2351821	+	1152	WP_011519195.1	polyphosphate kinase 2	-
LAI70_RS10395 (LAI70_10395)	2351998..2352282	+	285	WP_011519196.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LAI70_RS10400 (LAI70_10400)	2352292..2352639	+	348	WP_011519197.1	HigA family addiction module antitoxin	Antitoxin
LAI70_RS10405 (LAI70_10405)	2352754..2354067	+	1314	WP_011519198.1	hemolysin family protein	-
LAI70_RS10410 (LAI70_10410)	2354467..2355804	+	1338	WP_224444194.1	EAL domain-containing protein	-
LAI70_RS10415 (LAI70_10415)	2355964..2356077	+	114	WP_223277463.1	entericidin A/B family lipoprotein	-
LAI70_RS10420 (LAI70_10420)	2356196..2356837	-	642	WP_224444195.1	bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-99)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.15 Da
• Isoelectric Point: 8.5384

>T217156 WP_011519196.1 NZ_CP083718:2351998-2352282 [Cupriavidus metallidurans]
MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIKGPWHLCFRFI
NGEAWDVGIAEYEE

Nucleotide

• Length: 285 bp

>T217156 NZ_CP113925:219424-219687 [Bifidobacterium sp. ESL0728]
GTGACCTATCGCGTCGTCATCAAGAATTCAGCGAAGTCCGATCTTAAAAAACTCAAGCGTTCACATCTTCAGCAATCATT
CATGCAGGTCGTTGCGGATCTGAAATCAGATCCATATCAACCGACCCAATCATTCGAAAAGCTGCAGCCGACCAGCAGAG
GATTGTATTCTCGAAGGATCAACAGCCAACACCGTGTGGTTTACGATATCGATGACGATACGCAGACGGTAACGATATAT
GCAGCATGGAGCCATTACGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12691.46 Da
• Isoelectric Point: 7.3198

>AT217156 WP_011519197.1 NZ_CP083718:2352292-2352639 [Cupriavidus metallidurans]
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Nucleotide

• Length: 348 bp

>AT217156 NZ_CP113925:219164-219427 [Bifidobacterium sp. ESL0728]
ATGGATACCTATACTCCGACGGCGGCAAGGAAGAATCTGTATCAGATTCTCAAGGACGTCAACGTGCAGCGCAAGCCTGT
AGTTATCTCTCCCGCTAGGGGGAACAAGGACGAGGAAGCTGTGGTTGTCAATCGTGCCGATTGGAACTCGATTATGGAAA
CCCTCTATCTGGAAAACACCGGCACCCTCGCCACAGCTGAAAAGCGTCGTGCAGATGACAGCGGAACCACAGATATTGAT
GACATTGATTGGGACACGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A132H834