Detailed information of TA system TA217146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1263589..1264246
• Replicon: chromosome
• Accession: NZ_CP083709
• Organism: Enterobacter cloacae complex sp. ECL352

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A808IF03
• Locus tag: LBF07_RS06145
• Protein ID: WP_033146338.1
• Coordinates: 1263589..1263888 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LBF07_RS06150
• Protein ID: WP_094935697.1
• Coordinates: 1263899..1264246 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBF07_RS06130 (LBF07_06130)	1259513..1259962	+	450	Protein_1196	TolC family protein	-
LBF07_RS06135 (LBF07_06135)	1259959..1262157	+	2199	WP_112778387.1	type I secretion system permease/ATPase	-
LBF07_RS06140 (LBF07_06140)	1262168..1263337	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LBF07_RS06145 (LBF07_06145)	1263589..1263888	+	300	WP_033146338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LBF07_RS06150 (LBF07_06150)	1263899..1264246	+	348	WP_094935697.1	HigA family addiction module antitoxin	Antitoxin
LBF07_RS06155 (LBF07_06155)	1264291..1264716	+	426	WP_033146340.1	nucleoside triphosphatase NudI	-
LBF07_RS06160 (LBF07_06160)	1264688..1265419	-	732	WP_094935696.1	helix-turn-helix transcriptional regulator	-
LBF07_RS06165 (LBF07_06165)	1265517..1265936	+	420	WP_039263434.1	nuclear transport factor 2 family protein	-
LBF07_RS06170 (LBF07_06170)	1265939..1266457	-	519	WP_094935695.1	hypothetical protein	-
LBF07_RS06175 (LBF07_06175)	1266542..1267723	-	1182	WP_094935694.1	PLP-dependent aminotransferase family protein	-
LBF07_RS06180 (LBF07_06180)	1267744..1268631	-	888	WP_094935693.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1235085..1264246	29161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11691.32 Da
• Isoelectric Point: 10.0346

>T217146 WP_033146338.1 NZ_CP083709:1263589-1263888 [Enterobacter cloacae complex sp. ECL352]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T217146 NZ_CP113917:c257214-256957 [Bifidobacterium sp. ESL0775]
ATGAACCTCGCGTGGACCGAAGATGGCTGGAACGACTATCTGCACTGGCAACGGCAAGACAAGAAAACCCTCAGACGAAT
CAACACGCTTATCACGAACATGCTGAGAACCCCGTTCGAGGGCATCGGCAAGCCCGAGCCGCTGAAGTGGGGCCTGGAGG
GCGCTTGGTCGCGACGCATCGACAGCGCAAACCGCATCATCTACACCGTTTCCGACAACGAGATTTGTATTCTTTCGGCG
CGCAACCATTACGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13084.05 Da
• Isoelectric Point: 7.0134

>AT217146 WP_094935697.1 NZ_CP083709:1263899-1264246 [Enterobacter cloacae complex sp. ECL352]
MTLQQALRKPTTPGDVMQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT217146 NZ_CP113917:c257522-257211 [Bifidobacterium sp. ESL0775]
ATGGTGCAAGCAGTAGCGTATAGCAATTTTCGCAAGGACCTTAAATCATATATGCGCAAAGTCAACGAGGACGCGGACTT
GCTACTTATCACCAACCCGAATCCAGACGATAACGTGGTGGTGATGAGCGCCGACGACTACGAATCTTTGATGGAAACGA
TGCGCATCTACCAGAATCCCTATCTGCACAAAAAAATCACCAAAGGCTTGCAGCAGGTCAGGGACGGGAGCGTCCGGCAG
TACAGTCTCATCGATCCAGACGCTGAAGATTCTTATTCATCCCCAGCCAAGAGAAACGGAACGGAAGGATGA

Structures

Toxin

• AlphaFold DB: A0A808IF03