Detailed information of TA system TA217145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 639186..639846
• Replicon: chromosome
• Accession: NZ_CP083709
• Organism: Enterobacter cloacae complex sp. ECL352

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LBF07_RS03040
• Protein ID: WP_094933998.1
• Coordinates: 639493..639846 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LBF07_RS03035
• Protein ID: WP_094933997.1
• Coordinates: 639186..639488 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBF07_RS02995 (LBF07_02995)	634373..634666	-	294	WP_000034418.1	hypothetical protein	-
LBF07_RS03000 (LBF07_03000)	634757..635044	-	288	WP_016240042.1	hypothetical protein	-
LBF07_RS03005 (LBF07_03005)	635061..635447	-	387	WP_016240041.1	hypothetical protein	-
LBF07_RS03010 (LBF07_03010)	635462..635773	-	312	WP_001515481.1	hypothetical protein	-
LBF07_RS03015 (LBF07_03015)	635803..636018	-	216	WP_000250489.1	AlpA family transcriptional regulator	-
LBF07_RS03020 (LBF07_03020)	636205..637068	-	864	WP_016240040.1	DUF6387 family protein	-
LBF07_RS03025 (LBF07_03025)	637660..638049	-	390	WP_094933995.1	RidA family protein	-
LBF07_RS03030 (LBF07_03030)	638191..639111	+	921	WP_094933996.1	LysR family transcriptional regulator	-
LBF07_RS03035 (LBF07_03035)	639186..639488	-	303	WP_094933997.1	XRE family transcriptional regulator	Antitoxin
LBF07_RS03040 (LBF07_03040)	639493..639846	-	354	WP_094933998.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LBF07_RS03045 (LBF07_03045)	640023..640916	-	894	WP_094933999.1	LysR substrate-binding domain-containing protein	-
LBF07_RS03050 (LBF07_03050)	641090..641695	+	606	WP_224435145.1	glutathione S-transferase family protein	-
LBF07_RS03055 (LBF07_03055)	641739..642689	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
LBF07_RS03060 (LBF07_03060)	642786..643703	-	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13543.42 Da
• Isoelectric Point: 9.0310

>T217145 WP_094933998.1 NZ_CP083709:c639846-639493 [Enterobacter cloacae complex sp. ECL352]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
ILCAGNKVGNERRFYDEMLLVADREFTHWLNILKERE

Nucleotide

• Length: 354 bp

>T217145 NZ_CP113916:1113759-1114031 [Lactobacillus sp. ESL0785]
ATGTTAAATATTAAGGTTACAGCTAAGTTTAGGAAAGACGTCAAGCGGTGTAATAAGCAAGGGTTACCAATGACTCAACT
TAAGTTAGTTATTCATGAACTAGAACAAAATCATGTCCTGCCCTCTAATTATAAAAATCATGCTTTAGATGGAACTTACT
CTAATTATCTTGAATGTCATATAAAGCCAGATTGGTTGTTAATCTATCAAATTACTCAAACAACTTTGGCTCTTATTAGA
ACAGGCAGTCATGCAGAATTATTTAAAAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT217145 WP_094933997.1 NZ_CP083709:c639488-639186 [Enterobacter cloacae complex sp. ECL352]
MGRTLEQLIADEKPEVVASAQALATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT217145 NZ_CP113916:1113498-1113755 [Lactobacillus sp. ESL0785]
ATGAATAAAACTTTACAGGCCCGAATGGATGGTAAACTTATGGATGAGGCAGAAGGTATCTTAAAAGATTTAGGACTTAA
TCGTTCAACTGCCTTGAAAATTTTTTATCAACAAATTGTATGGAATAATGGGTTGCCTTTTGCAGTTAAAAAACCTGAAA
TCCCAAATGAAGAAACGTTAGATGCGATGCATGAAGATTTATCTAAGACTAAGACTTACGATAATTCAGATGAATTATGG
CATGACTTGGGCATTTAA