Detailed information of TA system TA217126

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36889..37153
• Replicon: plasmid p1606c
• Accession: NZ_CP083704
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K9O95_RS25860
• Protein ID: WP_001387489.1
• Coordinates: 37001..37153 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	36889..36949 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O95_RS28140 (32999)	32999..33277	+	279	WP_219334566.1	ash family protein	-
K9O95_RS25835 (33195)	33195..33482	+	288	WP_000074855.1	conjugal transfer protein TraA	-
K9O95_RS25840 (33924)	33924..34232	+	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
K9O95_RS25850 (34996)	34996..36153	-	1158	Protein_46	IS1380-like element ISEcp1 family transposase	-
K9O95_RS25855 (36306)	36306..36680	-	375	WP_223349261.1	hypothetical protein	-
- (36889)	36889..36949	-	61	NuclAT_0	-	Antitoxin
- (36889)	36889..36949	-	61	NuclAT_0	-	Antitoxin
- (36889)	36889..36949	-	61	NuclAT_0	-	Antitoxin
- (36889)	36889..36949	-	61	NuclAT_0	-	Antitoxin
K9O95_RS25860 (37001)	37001..37153	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
K9O95_RS25865 (37225)	37225..37476	-	252	WP_001291964.1	hypothetical protein	-
K9O95_RS25870 (37776)	37776..38072	+	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K9O95_RS25875 (38137)	38137..38313	-	177	WP_001054897.1	hypothetical protein	-
K9O95_RS25880 (38705)	38705..38914	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K9O95_RS25885 (38986)	38986..39084	-	99	Protein_53	ethanolamine utilization protein EutE	-
K9O95_RS25890 (39139)	39139..39836	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
K9O95_RS25895 (39852)	39852..39965	+	114	Protein_55	transposase	-
K9O95_RS25900 (40289)	40289..41434	+	1146	WP_075985684.1	class C beta-lactamase CMY-145	-
K9O95_RS25905 (41528)	41528..42061	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-145	-	1..58698	58698
	-	inside	IScluster/Tn	blaCMY-145	-	34665..41434	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T217126 WP_001387489.1 NZ_CP083704:37001-37153 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T217126 NZ_CP113865:c1728189-1727926 [Caldicellulosiruptor morganii]
ATGGGATATAAATTAAAGTTTTCAAAATATGCTTTAAAAGATGCAAAGAAACTAGAAGAATGCGGATTGGATAAAAAAGC
AAAGGAAATTTTAAGAATATTAAAAGAAAATCTATATCAAAATCCGCCACCTTATGAGAAATTAAAAGGAGAGCTAAGTG
GCAAGTTTTCAAGAAGAATAAACATACAGCATAGGATAATATACGAAGTTTTAGAAGAAGAAAAGATTGTTAAAATTTAT
AGGATGTGGACACACTACGAATAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT217126 NZ_CP083704:c36949-36889 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin