Detailed information of TA system TA217121

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70490..70911
• Replicon: plasmid p1606A
• Accession: NZ_CP083702
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K9O95_RS25000
• Protein ID: WP_096937776.1
• Coordinates: 70490..70615 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	70713..70911 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O95_RS24960 (65554)	65554..65769	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
K9O95_RS24965 (65905)	65905..66552	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
K9O95_RS24970 (66743)	66743..67126	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9O95_RS24975 (67496)	67496..68068	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
K9O95_RS24980 (68364)	68364..69185	-	822	WP_001234465.1	DUF932 domain-containing protein	-
K9O95_RS24985 (69304)	69304..69591	-	288	WP_000107242.1	hypothetical protein	-
K9O95_RS24990 (69616)	69616..69822	-	207	WP_000275854.1	hypothetical protein	-
K9O95_RS24995 (69892)	69892..70189	+	298	Protein_82	hypothetical protein	-
K9O95_RS25000 (70490)	70490..70615	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9O95_RS25005 (70557)	70557..70706	-	150	Protein_84	DUF5431 family protein	-
- (70713)	70713..70911	-	199	NuclAT_0	-	Antitoxin
- (70713)	70713..70911	-	199	NuclAT_0	-	Antitoxin
- (70713)	70713..70911	-	199	NuclAT_0	-	Antitoxin
- (70713)	70713..70911	-	199	NuclAT_0	-	Antitoxin
K9O95_RS25010 (70880)	70880..70985	-	106	Protein_85	plasmid SOS inhibition protein A	-
K9O95_RS25015 (71140)	71140..71457	+	318	Protein_86	IS110 family transposase	-
K9O95_RS25020 (71746)	71746..71997	-	252	Protein_87	iron-containing alcohol dehydrogenase	-
K9O95_RS25025 (72082)	72082..72594	-	513	WP_223350513.1	potassium channel family protein	-
K9O95_RS25030 (72721)	72721..73414	-	694	Protein_89	IS1 family transposase	-
K9O95_RS25035 (73471)	73471..73575	+	105	Protein_90	thiosulfate reductase cytochrome B subunit	-
K9O95_RS25040 (73821)	73821..75215	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / tet(A)	-	1..120731	120731
	-	flank	IS/Tn	-	-	72721..73098	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T217121 WP_096937776.1 NZ_CP083702:c70615-70490 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T217121 NZ_CP113834:c21786-21424 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT217121 NZ_CP083702:c70911-70713 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin