Detailed information of TA system TA217118

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29198..29452
• Replicon: plasmid p1606A
• Accession: NZ_CP083702
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: K9O95_RS24765
• Protein ID: WP_001367749.1
• Coordinates: 29198..29347 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29391..29452 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O95_RS24730 (25223)	25223..25624	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K9O95_RS24735 (25557)	25557..25814	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K9O95_RS24740 (25907)	25907..26560	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K9O95_RS24745 (26658)	26658..26798	-	141	WP_001333237.1	hypothetical protein	-
K9O95_RS24750 (27497)	27497..28354	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
K9O95_RS24755 (28347)	28347..28421	-	75	WP_001365705.1	RepA leader peptide Tap	-
K9O95_RS24760 (28657)	28657..28914	-	258	WP_000083833.1	replication regulatory protein RepA	-
K9O95_RS24765 (29198)	29198..29347	-	150	WP_001367749.1	Hok/Gef family protein	Toxin
- (29391)	29391..29452	+	62	NuclAT_1	-	Antitoxin
- (29391)	29391..29452	+	62	NuclAT_1	-	Antitoxin
- (29391)	29391..29452	+	62	NuclAT_1	-	Antitoxin
- (29391)	29391..29452	+	62	NuclAT_1	-	Antitoxin
K9O95_RS24770 (29591)	29591..30019	-	429	WP_029702151.1	hypothetical protein	-
K9O95_RS24775 (30232)	30232..30801	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
K9O95_RS24780 (30953)	30953..31579	-	627	WP_029702149.1	NYN domain-containing protein	-
K9O95_RS24785 (31991)	31991..32200	-	210	WP_039023209.1	hypothetical protein	-
K9O95_RS24790 (32331)	32331..32891	-	561	WP_001567328.1	fertility inhibition protein FinO	-
K9O95_RS24795 (32946)	32946..33692	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / tet(A)	-	1..120731	120731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T217118 WP_001367749.1 NZ_CP083702:c29347-29198 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T217118 NZ_CP113833:23132-23995 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT217118 NZ_CP083702:29391-29452 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin