Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4351920..4352647 Replicon chromosome
Accession NZ_CP083701
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K9O95_RS21305 Protein ID WP_000550189.1
Coordinates 4351920..4352234 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K9O95_RS21310 Protein ID WP_000560266.1
Coordinates 4352231..4352647 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS21285 (4348078) 4348078..4349064 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
K9O95_RS21290 (4349143) 4349143..4349835 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K9O95_RS21295 (4349912) 4349912..4350415 - 504 WP_001300832.1 M48 family metallopeptidase -
K9O95_RS21300 (4350500) 4350500..4351636 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K9O95_RS21305 (4351920) 4351920..4352234 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K9O95_RS21310 (4352231) 4352231..4352647 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K9O95_RS21315 (4352692) 4352692..4354710 - 2019 WP_064221460.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K9O95_RS21320 (4355036) 4355036..4357387 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T217113 WP_000550189.1 NZ_CP083701:4351920-4352234 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T217113 NZ_CP113832:c2612189-2612085 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
ACTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT217113 WP_000560266.1 NZ_CP083701:4352231-4352647 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT217113 NZ_CP113832:2612329-2612478 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCGATTGTATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References