Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2645734..2646275 Replicon chromosome
Accession NZ_CP083701
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag K9O95_RS13165 Protein ID WP_000615979.1
Coordinates 2645997..2646275 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K9O95_RS13160 Protein ID WP_000729704.1
Coordinates 2645734..2645994 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS13140 (2641409) 2641409..2642194 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
K9O95_RS13145 (2642166) 2642166..2643878 + 1713 Protein_2585 flagellar biosynthesis protein FlhA -
K9O95_RS13150 (2644102) 2644102..2644599 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K9O95_RS13155 (2644775) 2644775..2645548 - 774 WP_123297815.1 C40 family peptidase -
K9O95_RS13160 (2645734) 2645734..2645994 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K9O95_RS13165 (2645997) 2645997..2646275 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K9O95_RS13170 (2646431) 2646431..2647171 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K9O95_RS13175 (2647142) 2647142..2647909 - 768 WP_000333379.1 class II glutamine amidotransferase -
K9O95_RS13180 (2648115) 2648115..2648693 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T217109 WP_000615979.1 NZ_CP083701:2645997-2646275 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217109 NZ_CP113832:2315208-2315552 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ACGTAAAAGACCATGTGGATATAATGGAATTGTATAAACTTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAGTTTATTTGAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT217109 WP_000729704.1 NZ_CP083701:2645734-2645994 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217109 NZ_CP113832:2314857-2315189 [Enterococcus faecalis]
ATGAATACTTATGAAATAATAAAAGAGTTGACAAAAAGGAAGAAAATGTCTATTCGACAATTAGAAATTACTCTAGGTTA
CTCAAATGGATATTTTAGCAAGTGGAAAAAAGTTTCTCCAAACTCAGAAGGCCTACAAAAAGTTGCGGACTACTTCAATG
TATCGGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAATTCTGATGATTTAAATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATTCGTGCATATTTAAAGGGTAG
ATTCGGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References