Detailed information of TA system TA217108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2639537..2640231
• Replicon: chromosome
• Accession: NZ_CP083701
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: K9O95_RS13125
• Protein ID: WP_001263493.1
• Coordinates: 2639537..2639935 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: K9O95_RS13130
• Protein ID: WP_000554757.1
• Coordinates: 2639938..2640231 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (2635197)	2635197..2635277	-	81	NuclAT_11	-	-
- (2635197)	2635197..2635277	-	81	NuclAT_11	-	-
- (2635197)	2635197..2635277	-	81	NuclAT_11	-	-
- (2635197)	2635197..2635277	-	81	NuclAT_11	-	-
K9O95_RS13095 (2634537)	2634537..2635781	-	1245	WP_000189541.1	esterase FrsA	-
K9O95_RS13100 (2635873)	2635873..2636331	-	459	WP_061349133.1	xanthine phosphoribosyltransferase	-
K9O95_RS13105 (2636592)	2636592..2638049	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
K9O95_RS13110 (2638106)	2638106..2638627	-	522	Protein_2578	peptide chain release factor H	-
K9O95_RS13115 (2638626)	2638626..2638829	-	204	Protein_2579	RtcB family protein	-
K9O95_RS13120 (2639075)	2639075..2639527	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
K9O95_RS13125 (2639537)	2639537..2639935	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K9O95_RS13130 (2639938)	2639938..2640231	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K9O95_RS13135 (2640283)	2640283..2641338	-	1056	WP_001226164.1	DNA polymerase IV	-
K9O95_RS13140 (2641409)	2641409..2642194	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
K9O95_RS13145 (2642166)	2642166..2643878	+	1713	Protein_2585	flagellar biosynthesis protein FlhA	-
K9O95_RS13150 (2644102)	2644102..2644599	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T217108 WP_001263493.1 NZ_CP083701:c2639935-2639537 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217108 NZ_CP113832:497747-497875 [Enterococcus faecalis]
ATGAAAGGAGCAATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTAT
CTTCGTTATCTTAGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT217108 WP_000554757.1 NZ_CP083701:c2640231-2639938 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217108 NZ_CP113832:c497991-497786 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAA
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6