Detailed information of TA system TA217055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 43133..43784
• Replicon: plasmid unnamed2
• Accession: NZ_CP083660
• Organism: Acinetobacter variabilis strain FDAARGOS_1487

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9M950
• Locus tag: LA331_RS16505
• Protein ID: WP_005237491.1
• Coordinates: 43133..43489 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LA331_RS16510
• Protein ID: WP_005237493.1
• Coordinates: 43482..43784 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA331_RS16470 (LA331_16470)	38527..39093	+	567	WP_005237480.1	recombinase family protein	-
LA331_RS16475 (LA331_16475)	39315..39827	+	513	WP_005237482.1	hypothetical protein	-
LA331_RS16480 (LA331_16480)	39859..40062	+	204	WP_265596773.1	DUF4145 domain-containing protein	-
LA331_RS16485 (LA331_16485)	40251..41069	-	819	WP_005237486.1	acetoacetate decarboxylase family protein	-
LA331_RS16490 (LA331_16490)	41220..41363	-	144	Protein_41	transposase	-
LA331_RS16495 (LA331_16495)	41464..41949	-	486	WP_005237487.1	GNAT family N-acetyltransferase	-
LA331_RS16500 (LA331_16500)	42033..42506	-	474	WP_005237489.1	GNAT family N-acetyltransferase	-
LA331_RS16505 (LA331_16505)	43133..43489	+	357	WP_005237491.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA331_RS16510 (LA331_16510)	43482..43784	+	303	WP_005237493.1	XRE family transcriptional regulator	Antitoxin
LA331_RS16515 (LA331_16515)	43826..43987	-	162	Protein_46	IS6 family transposase	-
LA331_RS16520 (LA331_16520)	44081..44473	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
LA331_RS16525 (LA331_16525)	44563..45843	+	1281	WP_005237495.1	cation transporter	-
LA331_RS16530 (LA331_16530)	45929..46633	+	705	WP_005237497.1	IS6-like element IS1006 family transposase	-
LA331_RS16535 (LA331_16535)	47853..48467	+	615	WP_004281236.1	thiol:disulfide interchange protein DsbA/DsbL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89812	89812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13596.61 Da
• Isoelectric Point: 7.1651

>T217055 WP_005237491.1 NZ_CP083660:43133-43489 [Acinetobacter variabilis]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKEVRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T217055 NZ_CP113784:1355575-1355949 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11208.99 Da
• Isoelectric Point: 6.4759

>AT217055 WP_005237493.1 NZ_CP083660:43482-43784 [Acinetobacter variabilis]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTLKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT217055 NZ_CP113784:1355333-1355575 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: N9M950