Detailed information of TA system TA217036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3425491..3426307
• Replicon: chromosome
• Accession: NZ_CP083651
• Organism: Citrobacter amalonaticus strain FDAARGOS_1489

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: LA348_RS16275
• Protein ID: WP_061069544.1
• Coordinates: 3425491..3425967 (-)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A3Q8DNJ3
• Locus tag: LA348_RS16280
• Protein ID: WP_042999108.1
• Coordinates: 3425972..3426307 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA348_RS16255 (LA348_16255)	3420596..3421339	-	744	WP_044264050.1	molecular chaperone	-
LA348_RS16260 (LA348_16260)	3421382..3423826	-	2445	WP_224267598.1	fimbria/pilus outer membrane usher protein	-
LA348_RS16265 (LA348_16265)	3423920..3424468	-	549	WP_042999111.1	fimbrial protein	-
LA348_RS16270 (LA348_16270)	3424537..3425067	-	531	WP_042999110.1	fimbrial protein	-
LA348_RS16275 (LA348_16275)	3425491..3425967	-	477	WP_061069544.1	type II toxin-antitoxin system YhaV family toxin	Toxin
LA348_RS16280 (LA348_16280)	3425972..3426307	-	336	WP_042999108.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
LA348_RS16285 (LA348_16285)	3426522..3427058	-	537	WP_042999107.1	ferredoxin-type protein NapF	-
LA348_RS16290 (LA348_16290)	3427055..3427651	-	597	WP_042999106.1	molecular chaperone	-
LA348_RS16295 (LA348_16295)	3427696..3428469	-	774	WP_044257303.1	dimethyl sulfoxide reductase anchor subunit	-
LA348_RS16300 (LA348_16300)	3428462..3429088	-	627	WP_042999104.1	dimethylsulfoxide reductase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (15-110)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18194.81 Da
• Isoelectric Point: 8.4404

>T217036 WP_061069544.1 NZ_CP083651:c3425967-3425491 [Citrobacter amalonaticus]
MDFPTWLNGWTIYAHPCFIDQYNALVARVEDLKHRFPDPIVFQKKKETKVLAHLLKSIANITHEPRASVYLPGDSIGKAY
TDWSRAKFGGGRYRLFFRYSLESKIIVIAWVNDEGPLRTYGSKTDAYKIFGKMLDEGNPPDDWLSLLQACQNDGKEHL

Nucleotide

• Length: 477 bp

>T217036 NZ_CP113541:c1024869-1024342 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.83 Da
• Isoelectric Point: 4.1876

>AT217036 WP_042999108.1 NZ_CP083651:c3426307-3425972 [Citrobacter amalonaticus]
MFAYVRSEDIAIEADSKLTERHQTTIPASIRDALHLRSGDRIHYTLLSTGEVLLSKYSEATEDGVMRSFLDFLSQDVANN
PQTLKKLDLSRGYELVDGIDVNLDDEISDED

Nucleotide

• Length: 336 bp

>AT217036 NZ_CP113541:c1025156-1024866 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DNJ3