Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1862805..1863381 Replicon chromosome
Accession NZ_CP083648
Organism Corynebacterium coyleae strain FDAARGOS_1492

Toxin (Protein)


Gene name graT Uniprot ID D7WDU2
Locus tag LA324_RS08750 Protein ID WP_005290974.1
Coordinates 1863100..1863381 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F1URP9
Locus tag LA324_RS08745 Protein ID WP_070452758.1
Coordinates 1862805..1863065 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA324_RS08705 (LA324_08705) 1858127..1859158 + 1032 WP_224212869.1 HAMP domain-containing sensor histidine kinase -
LA324_RS08710 (LA324_08710) 1859155..1859601 - 447 WP_070819876.1 aminoglycoside N-acetyltransferase AAC(3)-XI -
LA324_RS08715 (LA324_08715) 1859739..1860776 + 1038 WP_092102067.1 FUSC family protein -
LA324_RS08720 (LA324_08720) 1860773..1861009 - 237 WP_070452630.1 hypothetical protein -
LA324_RS08725 (LA324_08725) 1861175..1861390 + 216 WP_070452628.1 DUF6290 family protein -
LA324_RS08730 (LA324_08730) 1861400..1861687 + 288 WP_070452625.1 type II toxin-antitoxin system RelE/ParE family toxin -
LA324_RS08735 (LA324_08735) 1861709..1862152 + 444 WP_070452622.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
LA324_RS08740 (LA324_08740) 1862201..1862791 + 591 WP_083284865.1 putative glycolipid-binding domain-containing protein -
LA324_RS08745 (LA324_08745) 1862805..1863065 - 261 WP_070452758.1 HigA family addiction module antitoxin Antitoxin
LA324_RS08750 (LA324_08750) 1863100..1863381 - 282 WP_005290974.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LA324_RS08755 (LA324_08755) 1863715..1864065 + 351 WP_070452620.1 VOC family protein -
LA324_RS08760 (LA324_08760) 1864268..1864396 + 129 WP_005293248.1 monooxygenase -
LA324_RS08765 (LA324_08765) 1864440..1864829 - 390 WP_005290994.1 heavy metal-responsive transcriptional regulator -
LA324_RS08770 (LA324_08770) 1864928..1866349 + 1422 WP_224212870.1 mercury(II) reductase -
LA324_RS08775 (LA324_08775) 1866346..1866696 + 351 WP_038593990.1 hypothetical protein -
LA324_RS08780 (LA324_08780) 1866889..1867158 + 270 WP_005293257.1 hypothetical protein -
LA324_RS08785 (LA324_08785) 1867321..1867443 + 123 Protein_1701 IS3 family transposase -
LA324_RS08790 (LA324_08790) 1867577..1868200 - 624 WP_224212871.1 ATP-binding cassette domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1861709..1870594 8885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(2-69)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11051.53 Da        Isoelectric Point: 9.8469

>T217023 WP_005290974.1 NZ_CP083648:c1863381-1863100 [Corynebacterium coyleae]
MIQSFADKDTERLWNRERVRSIDSRIHSVALRKLRQLGYAQTLDELRIPPGNRLEALKGDRRGQFSIRINDQWRICFRWS
AAGPEEVEIVDYH

Download         Length: 282 bp

>T217023 NZ_CP113540:3446165-3446383 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9677.22 Da        Isoelectric Point: 5.6726

>AT217023 WP_070452758.1 NZ_CP083648:c1863065-1862805 [Corynebacterium coyleae]
MEDFLKEMEITQHKLAVSIGVPPRRINEIVHGKRAVTADTALRLAKFFGMSPQFWLGLQAQYDLDVAEDKILAEIERIQP
LQAASA

Download         Length: 261 bp

>AT217023 NZ_CP113540:3445763-3446137 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F1ZH13

Antitoxin

Source ID Structure
AlphaFold DB A0A1F1URP9

References