Detailed information of TA system TA216969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2999348..3000147
• Replicon: chromosome
• Accession: NZ_CP083638
• Organism: Escherichia fergusonii strain FDAARGOS_1499

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7LMV3
• Locus tag: LA347_RS14525
• Protein ID: WP_000347277.1
• Coordinates: 2999683..3000147 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7LMV4
• Locus tag: LA347_RS14520
• Protein ID: WP_015953962.1
• Coordinates: 2999348..2999683 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA347_RS14505 (2995472)	2995472..2996362	-	891	WP_000178101.1	2-hydroxy-3-oxopropionate reductase	-
LA347_RS14510 (2996387)	2996387..2997157	-	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
LA347_RS14515 (2997628)	2997628..2999199	+	1572	WP_000247251.1	galactarate dehydratase	-
LA347_RS14520 (2999348)	2999348..2999683	+	336	WP_015953962.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LA347_RS14525 (2999683)	2999683..3000147	+	465	WP_000347277.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LA347_RS14530 (3000202)	3000202..3001011	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LA347_RS14535 (3001259)	3001259..3002539	+	1281	WP_000681964.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LA347_RS14540 (3002562)	3002562..3003035	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LA347_RS14545 (3003046)	3003046..3003825	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LA347_RS14550 (3003815)	3003815..3004693	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
LA347_RS14555 (3004711)	3004711..3005145	+	435	WP_000948832.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T216969 WP_000347277.1 NZ_CP083638:2999683-3000147 [Escherichia fergusonii]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T216969 NZ_CP113536:3515535-3515753 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12281.89 Da
• Isoelectric Point: 4.4128

>AT216969 WP_015953962.1 NZ_CP083638:2999348-2999683 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT216969 NZ_CP113536:3515133-3515507 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: B7LMV3

Antitoxin

• AlphaFold DB: B7LMV4