Detailed information of TA system TA216965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2344869..2345548
• Replicon: chromosome
• Accession: NZ_CP083638
• Organism: Escherichia fergusonii strain FDAARGOS_1499

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: LA347_RS11265
• Protein ID: WP_002431667.1
• Coordinates: 2345246..2345548 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LA347_RS11260
• Protein ID: WP_000806441.1
• Coordinates: 2344869..2345210 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA347_RS11250 (2340655)	2340655..2341944	-	1290	WP_000102764.1	APC family permease	-
LA347_RS11255 (2342251)	2342251..2344755	+	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
LA347_RS11260 (2344869)	2344869..2345210	-	342	WP_000806441.1	HigA family addiction module antitoxin	Antitoxin
LA347_RS11265 (2345246)	2345246..2345548	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA347_RS11270 (2345681)	2345681..2346475	+	795	WP_000365145.1	TraB/GumN family protein	-
LA347_RS11275 (2346679)	2346679..2347158	+	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
LA347_RS11280 (2347182)	2347182..2347982	+	801	WP_000439795.1	hypothetical protein	-
LA347_RS11285 (2347979)	2347979..2348482	+	504	WP_000667001.1	hypothetical protein	-
LA347_RS11290 (2348520)	2348520..2350172	-	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T216965 WP_002431667.1 NZ_CP083638:c2345548-2345246 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T216965 NZ_CP113536:883079-883477 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT216965 WP_000806441.1 NZ_CP083638:c2345210-2344869 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT216965 NZ_CP113536:882852-883079 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8