Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1352821..1353383 Replicon chromosome
Accession NZ_CP083638
Organism Escherichia fergusonii strain FDAARGOS_1499

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag LA347_RS06590 Protein ID WP_000605676.1
Coordinates 1352821..1353099 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag LA347_RS06595 Protein ID WP_000781370.1
Coordinates 1353099..1353383 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA347_RS06565 (1348409) 1348409..1348840 - 432 WP_000152299.1 peroxiredoxin OsmC -
LA347_RS06570 (1349183) 1349183..1349398 + 216 WP_000495766.1 biofilm-dependent modulation protein -
LA347_RS06575 (1349500) 1349500..1349637 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
LA347_RS06580 (1349794) 1349794..1351491 + 1698 WP_000433464.1 malate dehydrogenase -
LA347_RS06585 (1351625) 1351625..1352635 + 1011 WP_000642397.1 alcohol dehydrogenase AdhP -
LA347_RS06590 (1352821) 1352821..1353099 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LA347_RS06595 (1353099) 1353099..1353383 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
LA347_RS06600 (1353434) 1353434..1354087 - 654 WP_000045645.1 formate dehydrogenase-N subunit gamma -
LA347_RS06605 (1354080) 1354080..1354964 - 885 WP_001240580.1 formate dehydrogenase N subunit beta -
LA347_RS06610 (1354977) 1354977..1358024 - 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T216956 WP_000605676.1 NZ_CP083638:1352821-1353099 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T216956 NZ_CP113535:c4191274-4190987 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT216956 WP_000781370.1 NZ_CP083638:1353099-1353383 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT216956 NZ_CP113535:c4191000-4190698 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References