Detailed information of TA system TA216953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 81240..81934
• Replicon: chromosome
• Accession: NZ_CP083638
• Organism: Escherichia fergusonii strain FDAARGOS_1499

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: B7LNG9
• Locus tag: LA347_RS00350
• Protein ID: WP_001263502.1
• Coordinates: 81536..81934 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LA347_RS00345
• Protein ID: WP_000554758.1
• Coordinates: 81240..81533 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA347_RS00330 (77593)	77593..79296	-	1704	Protein_65	flagellar biosynthesis protein FlhA	-
LA347_RS00335 (79277)	79277..80062	+	786	WP_000207582.1	putative lateral flagellar export/assembly protein LafU	-
LA347_RS00340 (80133)	80133..81188	+	1056	WP_001226166.1	DNA polymerase IV	-
LA347_RS00345 (81240)	81240..81533	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LA347_RS00350 (81536)	81536..81934	+	399	WP_001263502.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LA347_RS00355 (81944)	81944..82396	+	453	WP_001059849.1	GNAT family N-acetyltransferase	-
LA347_RS00360 (82714)	82714..83853	+	1140	WP_000521571.1	RNA ligase RtcB family protein	-
LA347_RS00365 (83850)	83850..84464	+	615	WP_000602110.1	peptide chain release factor H	-
LA347_RS00370 (84526)	84526..85050	+	525	WP_024256511.1	metal-dependent hydrolase	-
LA347_RS00375 (85059)	85059..85301	-	243	WP_001029361.1	hypothetical protein	-
LA347_RS00380 (85349)	85349..86806	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15486.90 Da
• Isoelectric Point: 8.9401

>T216953 WP_001263502.1 NZ_CP083638:81536-81934 [Escherichia fergusonii]
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T216953 NZ_CP113535:2485142-2485426 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT216953 WP_000554758.1 NZ_CP083638:81240-81533 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT216953 NZ_CP113535:2484904-2485152 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: B7LNG9

Antitoxin

• AlphaFold DB: S1QAE3