Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1115167..1115894 Replicon chromosome
Accession NZ_CP083630
Organism Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)


Gene name higB Uniprot ID A0A4V6JIZ2
Locus tag LA332_RS05430 Protein ID WP_032611627.1
Coordinates 1115583..1115894 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2C5SAL7
Locus tag LA332_RS05425 Protein ID WP_032611629.1
Coordinates 1115167..1115586 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA332_RS05405 (LA332_05405) 1110640..1113756 + 3117 WP_032611637.1 multidrug efflux RND transporter permease subunit KexD -
LA332_RS05410 (LA332_05410) 1113832..1114368 + 537 WP_032611635.1 GNAT family protein -
LA332_RS05415 (LA332_05415) 1114491..1114703 + 213 Protein_1052 zinc ABC transporter substrate-binding protein -
LA332_RS05420 (LA332_05420) 1114958..1115140 + 183 WP_032611631.1 hypothetical protein -
LA332_RS05425 (LA332_05425) 1115167..1115586 - 420 WP_032611629.1 helix-turn-helix domain-containing protein Antitoxin
LA332_RS05430 (LA332_05430) 1115583..1115894 - 312 WP_032611627.1 type II toxin-antitoxin system HigB family toxin Toxin
LA332_RS05435 (LA332_05435) 1116955..1117359 - 405 WP_032611624.1 H-NS family nucleoid-associated regulatory protein -
LA332_RS05440 (LA332_05440) 1119305..1119718 + 414 WP_032611622.1 hypothetical protein -
LA332_RS05445 (LA332_05445) 1119869..1120566 + 698 WP_224214505.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12431.23 Da        Isoelectric Point: 9.8984

>T216918 WP_032611627.1 NZ_CP083630:c1115894-1115583 [Leclercia adecarboxylata]
MHVISKEPFEEAVKRYPNDSLAIRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDIGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T216918 NZ_CP113517:3150776-3151030 [Methylomonas rapida]
ATGGATACCATCAATTACACAACCTTGCGCAACAACCTCGCCAGCGTGCTTGATAAAGTCAACGACGACCATAACCCGGT
CTTGGTGACACGGCAAAATGGCAAACCGGCGGTGATCTTGTCGCTGGAAGATTACAAATCCTTCGAGGAAACCGCTTATT
TGATGGCCAGCCCGGTCAATGCGCAGCGTTTGAACCAAGCAATTGCGGAAGTGGAAGCCGGTAATACCGTAAACCATGAA
CTGATAGAACCATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15397.51 Da        Isoelectric Point: 4.4202

>AT216918 WP_032611629.1 NZ_CP083630:c1115586-1115167 [Leclercia adecarboxylata]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIANDDENPLIDFLASKIADYEDNSPRFAEFNKAVAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFF

Download         Length: 420 bp

>AT216918 NZ_CP113517:3151027-3151281 [Methylomonas rapida]
ATGATTTTGGCTTGGGCGACAAATGCTTGGGACGATTACTTGTACTGGCAGGCCACCGATAAAAAGGTGCTGAAACGTAT
CAATACCTTGATCGAAGACATCAAACGCCAGCCATTCACCGGCATTGGCGATCCGGAGCTATTAAAGCACAACTGGTCCG
GATACTGGTCCAGGCGCATCGACCGTGAACATCGGTTGGTTTACAAGGCGGATGCCACCACGATTACCATCGTGCAATGT
CGCTATCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4V6JIZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2C5SAL7

References